Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai, China.
Typhoon Institute/CMA, Shanghai Key Laboratory of Meteorology and Health, Shanghai, 200030, China.
Sci Total Environ. 2020 Apr 20;714:136790. doi: 10.1016/j.scitotenv.2020.136790. Epub 2020 Jan 18.
Ambient fine particulate matter (PM) exposure has been linked with various adverse health outcomes. However, the urine metabolomics changes impacted by PM have not been well elucidated.
The normal healthy C57BL/6 mice were exposed to concentrated ambient PM (PM) or filtered air (FA) for four weeks using "Shanghai-METAS". The urinary metabolome was quantified using liquid/gas chromatography coupled with mass spectrometry.
There were 2213 metabolites identified in total and 163 of them were significantly different between FA- and PM-exposed mice. The KEGG pathway analysis suggested that there were nine perturbed metabolic pathways related to amino acid metabolism. The amino acid metabolism what mainly impacted by PM were beta-alanine, arginine, proline, alanine, aspartate, glutamate, phenylalanine, glycine, serine, threonine and tyrosine metabolism. Meanwhile, nineteen differential metabolites related to lipid metabolism and seven differential metabolites related to glucose homeostasis were different between FA and PM mice. Furthermore, the glucose and its metabolites were significantly increased in the PM mice compared with the FA mice.
The current study provided a critical information on evaluating the systemic toxicity of PM. The results demonstrated that there were significant alterations in urine metabolome by short-term exposure to PM, including amino acid metabolism, lipid metabolism and glucose metabolism. The metabolomics approach might be an effective tool to evaluate the potential mechanism of PM in inducing adverse health outcomes.
环境细颗粒物（PM）暴露与各种健康不良后果有关。然而，PM 影响的尿液代谢组学变化尚未得到充分阐明。
使用“上海-METAS”将正常健康的 C57BL/6 小鼠暴露于浓缩环境 PM（PM）或过滤空气（FA）中四周。使用液相/气相色谱-质谱联用技术定量尿液代谢组。
总共鉴定出 2213 种代谢物，其中 163 种在 FA 和 PM 暴露的小鼠之间存在显著差异。KEGG 途径分析表明，有 9 个与氨基酸代谢相关的失调代谢途径。PM 主要影响的氨基酸代谢途径为β-丙氨酸、精氨酸、脯氨酸、丙氨酸、天冬氨酸、谷氨酸、苯丙氨酸、甘氨酸、丝氨酸、苏氨酸和酪氨酸代谢。同时，FA 和 PM 小鼠之间有 19 种差异代谢物与脂质代谢有关，7 种差异代谢物与葡萄糖稳态有关。此外，与 FA 小鼠相比，PM 小鼠的葡萄糖及其代谢物明显增加。
本研究提供了评估 PM 全身毒性的重要信息。结果表明，短期暴露于 PM 会导致尿液代谢组发生显著变化，包括氨基酸代谢、脂质代谢和葡萄糖代谢。代谢组学方法可能是评估 PM 诱导不良健康后果的潜在机制的有效工具。
