Xu Xihui, Xu Meng, Zhao Qiming, Xia Yan, Chen Chen, Shen Zhenguo
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Curr Microbiol. 2018 Sep;75(9):1231-1239. doi: 10.1007/s00284-018-1515-z. Epub 2018 May 26.
Microbe-assisted phytoremediation has great potential for practical applications. Plant growth-promoting bacteria (PGPB) with heavy metal (HM) resistance are important for the implementation of PGPB-assisted phytoremediation of HM-contaminated environments. Arthrobacter sp. PGP41 is a Cd(II)-resistant bacterium isolated from the rhizosphere soils of a Cd(II) hyperaccumulator plant, Solanum nigrum. Strain PGP41 can significantly improve plant seedling and root growth under Cd(II) stress conditions. This bacterium exhibited the ability to produce high levels of indole-3-acetic acid (IAA), as well as the ability to fix nitrogen and solubilize phosphate, and it possessed 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. Here, we present the complete genome sequence of strain PGP41. The genome consists of a single chromosome with a G+C content of 65.38% and no plasmids. The genome encodes 3898 genes and contains 49 tRNA and 12 rRNA genes. Multiple genes associated with plant growth promotion were identified in the genome. The whole genome sequence of PGP41 provides information useful for further clarifying the molecular mechanisms behind plant growth promotion by PGPB and facilitates its potential use as an inoculum in the bioremediation of HM-contaminated environments.
微生物辅助植物修复具有巨大的实际应用潜力。具有重金属抗性的植物促生细菌（PGPB）对于实施PGPB辅助的重金属污染环境植物修复至关重要。节杆菌属PGP41是从镉超积累植物龙葵根际土壤中分离出的一种抗镉（II）细菌。PGP41菌株在镉（II）胁迫条件下能显著促进植物幼苗和根系生长。该细菌具有产生高水平吲哚-3-乙酸（IAA）的能力，以及固氮和溶解磷酸盐的能力，并且具有1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性。在此，我们展示了PGP41菌株的完整基因组序列。该基因组由一条单染色体组成，G+C含量为65.38%，无质粒。基因组编码3898个基因，包含49个tRNA和12个rRNA基因。在基因组中鉴定出多个与植物生长促进相关的基因。PGP41的全基因组序列为进一步阐明PGPB促进植物生长的分子机制提供了有用信息，并有助于其作为接种剂在重金属污染环境生物修复中的潜在应用。
