Yang Lei-Lei, Xin Yu-Hua, Liu Qing
State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, NO. 1 West Beichen, Chaoyang District, Beijing, 100101, People's Republic of China.
Beijing Key Laboratory of Genetic Element Biosourcing & Intelligent Design for Biomanufacturing, Beijing, 100101, People's Republic of China.
Curr Microbiol. 2025 Aug 18;82(10):462. doi: 10.1007/s00284-025-04432-6.
In a study on bacterial diversity on glacier surfaces in China, five bacterial strains (HLT1-5, HLT3-5, MDT3-26, MDT3-9, and MDT3-28) were isolated from soil samples collected from the surfaces of Hailuogou glacier and Midui glacier. Phylogenetic analysis based on 16S rRNA gene sequences placed these strains close to Pseudarthrobacter humi RMG13, Pseudarthrobacter psychrotolerans YJ56, and Pseudarthrobacter sulfonivorans ALL, with sequence similarity ranging from 98.98 to 100%. The phylogenomic tree further confirmed these relationships. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between these five strains and other members of the genus Pseudarthrobacter were both lower than 95% and 70%, respectively, indicating that they represent two novel species. The draft genomes contained 4162-4359 genes, including several resistance genes for heavy metals. They exhibited varying levels of resistance to heavy metals such as Cu⁺, Co⁺, and Zn⁺. Genomic data indicated that the strains harbored copper-exporting ATPase genes, multi-copper oxidases, and other key genes related to copper, cobalt, zinc, and cadmium resistance. Based on polyphasic characterizations, strains MDT3-26, MDT3-28, and MDT3-9 represent a novel species of the genus Pseudarthrobacter, for which the name Pseudarthrobacter raffinosi sp. nov. is proposed, with the type strain MDT3-26 (= CGMCC 1.9918 = NBRC 113090). Strains HLT1-5 and HLT3-5 represent another novel species, for which the name Pseudarthrobacter cellobiosi sp. nov. is proposed, with the type strain HLT1-5 (= CGMCC 1.9998 = NBRC 113091). These findings characterize two novel species within the genus Pseudarthrobacter and provide new insights into the bioremediation potential of this genus.
在中国一项关于冰川表面细菌多样性的研究中，从海螺沟冰川和米堆冰川表面采集的土壤样本中分离出了五株细菌菌株（HLT1 - 5、HLT3 - 5、MDT3 - 26、MDT3 - 9和MDT3 - 28）。基于16S rRNA基因序列的系统发育分析表明，这些菌株与土壤假节杆菌RMG13、耐冷假节杆菌YJ56和嗜硫假节杆菌ALL亲缘关系较近，序列相似性在98.98%至100%之间。系统发育基因组树进一步证实了这些关系。这五株菌株与假节杆菌属其他成员之间的平均核苷酸同一性（ANI）和数字DNA - DNA杂交（dDDH）值分别低于95%和70%，表明它们代表两个新物种。草图基因组包含4162 - 4359个基因，其中包括几个重金属抗性基因。它们对Cu⁺、Co⁺和Zn⁺等重金属表现出不同程度的抗性。基因组数据表明，这些菌株含有铜输出ATP酶基因、多铜氧化酶以及其他与铜、钴、锌和镉抗性相关的关键基因。基于多相特征分析，菌株MDT3 - 26、MDT3 - 28和MDT3 - 9代表假节杆菌属的一个新物种，为此提出新名称嗜棉籽糖假节杆菌（Pseudarthrobacter raffinosi sp. nov.），模式菌株为MDT3 - 26（= CGMCC 1.9918 = NBRC 113090）。菌株HLT1 - 5和HLT3 - 5代表另一个新物种，为此提出新名称嗜纤维二糖假节杆菌（Pseudarthrobacter cellobiosi sp. nov.），模式菌株为HLT1 - 5（= CGMCC 1.9998 = NBRC 113091）。这些发现鉴定了假节杆菌属内的两个新物种，并为该属的生物修复潜力提供了新的见解。
