Miao R, Dong X B, Gong J N, Li J F, Pang W Y, Liu Y Y, Yang Y H
Medical Research Center, Beijing Chaoyang Hospital, Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Capital Medical University, Beijing 100020, China.
Zhonghua Yi Xue Za Zhi. 2018 May 15;98(18):1397-1402. doi: 10.3760/cma.j.issn.0376-2491.2018.18.006.
To find key microRNA (miR) associated with chronic thromboembolic pulmonary hypertension (CTEPH). Affymetrix miR microarray data and GSE56914 data downloaded from GEO database (http: //www.ncbi.nlm.nih.gov/geo/) were obtained and integrated. The microarray data were obtained from peripheral blood samples of CTEPH patients and the matched control. Differentially expressed miRs were screened. Target genes of these miRs were searched. Then, functional enrichment analyses for these miRs were performed. After that, disease network including miRs, target genes and pathways was constructed. Five important miRs including hsa-miR-885-5p, hsa-miR-501-5p, hsa-miR-615-3p, hsa-miR-610, and hsa-miR-346 were identified. Furthermore, hsa-miR-885-5p and hsa-miR-501-5p were significantly enriched in cell cycle pathway. Hsa-miR-615-3p was involved in cytokine-cytokine receptor interaction, axon guidance, focal adhesion and cell cycle pathway. Hsa-miR-610 was significantly enriched in focal adhesion pathway, and hsa-miR-346 was involved in cytokine-cytokine receptor interaction, axon guidance, and focal adhesion pathway. Hsa-miR-885-5p, hsa-miR-501-5p, hsa-miR-615-3p, hsa-miR-610 and hsa-miR-346 are important miRs for the development of CTEPH.
寻找与慢性血栓栓塞性肺动脉高压（CTEPH）相关的关键微小RNA（miR）。获取并整合从基因表达综合数据库（GEO数据库，网址：http://www.ncbi.nlm.nih.gov/geo/）下载的Affymetrix miR微阵列数据和GSE56914数据。微阵列数据取自CTEPH患者的外周血样本及匹配的对照。筛选差异表达的miR，搜索这些miR的靶基因，然后对这些miR进行功能富集分析。之后，构建包括miR、靶基因和通路的疾病网络。鉴定出5个重要的miR，分别为hsa-miR-885-5p、hsa-miR-501-5p、hsa-miR-615-3p、hsa-miR-610和hsa-miR-346。此外，hsa-miR-885-5p和hsa-miR-501-5p在细胞周期通路中显著富集。hsa-miR-615-3p参与细胞因子-细胞因子受体相互作用、轴突导向、粘着斑和细胞周期通路。hsa-miR-610在粘着斑通路中显著富集，hsa-miR-346参与细胞因子-细胞因子受体相互作用、轴突导向和粘着斑通路。hsa-miR-885-5p、hsa-miR-501-5p、hsa-miR-615-3p、hsa-miR-610和hsa-miR-346是CTEPH发生发展的重要miR。
