College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
Carbohydr Polym. 2018 Sep 1;195:275-287. doi: 10.1016/j.carbpol.2018.04.109. Epub 2018 Apr 30.
This work aims to synthesize poly(aminoethyl) modified chitin (PAEMC) and ascertain its antibacterial activity and mechanism. FTIR and H NMR results proved aminoethyl moieties were grafted to C6OH and C3OH on chitin backbone in the form of polymerization. XRD and TG/DTG analyses manifested its well-defined crystallinity and thermostability. PAEMC, with average molecular weight (MW) of 851.0 kDa, degree of deacetylation (DD) of 27.95%, and degree of substitution (DS) of 1.77, had good solubility in aqueous solutions over the pH range of 3-12, and also possessed high antimicrobial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus proteus, and Klebsiella pneumoniae, commonly causing chronic wound infections. Nucleic acid release, protein leakage, increased inner membrane permeability, and decreased cell surface hydrophobicity implied bacterial cytomembranes were substantially compromised in the presence of PAEMC. Microscopically, PAEMC visually perturbed bacteria, illustrating deformed and even collapsed morphologies. Overall, PAEMC possessed good solubility, effectively destroyed bacteria via a membrane damage mechanism, and might serve as an antibacterial agent for treatments of chronic wound infections.
本工作旨在合成聚（氨基乙基）改性壳聚糖（PAEMC），并确定其抗菌活性和机制。FTIR 和 H NMR 结果证明，氨基乙基基团以聚合的形式接枝到壳聚糖主链上的 C6OH 和 C3OH 上。XRD 和 TG/DTG 分析表明其具有明确的结晶度和热稳定性。PAEMC 的重均分子量（MW）为 851.0 kDa，脱乙酰度（DD）为 27.95%，取代度（DS）为 1.77，在 pH 值为 3-12 的水溶液中具有良好的溶解性，对表皮葡萄球菌、金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、变形杆菌和肺炎克雷伯菌等常见慢性伤口感染菌具有较高的抗菌活性。核酸释放、蛋白质渗漏、内膜通透性增加和细胞表面疏水性降低表明，PAEMC 严重破坏了细菌的细胞质膜。显微镜下，PAEMC 明显扰乱了细菌，呈现出变形甚至崩溃的形态。总的来说，PAEMC 具有良好的溶解性，通过破坏细胞膜的机制有效地杀死细菌，可能作为治疗慢性伤口感染的抗菌剂。
