The Antibacterial Activity and Mechanism of Action of Luteolin Against .

PURPOSE

This research aimed to investigate the antibacterial activity and potential mechanism of luteolin against .

MATERIALS AND METHODS

The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of luteolin against various strains. The potential mechanism of action of luteolin was elucidated through testing and analysing the luteolin-induced alterations of in several aspects, including cell wall, cell membrane, protein expression, nucleic acid content, topoisomerase activity and energy metabolism.

RESULTS

The MIC values of luteolin against various isolates and ATCC19411 were 78 µg/mL. The increased cell membrane permeability, destruction of cell wall integrity and TEM images after exposure to luteolin showed that the cell wall and membrane were damaged. The content of total protein and nucleic acid in decreased significantly after treatment with luteolin (1/2 MIC) for 12, 24, and 36 h. Moreover, a hypochromic effect was observed in the absorption spectrum of luteolin when deoxyribonucleic acid (DNA) was added. In addition, after treatment with luteolin, a decrease in nicked or relaxed DNA content, which was catalysed by -isolated DNA topoisomerase, was observed. In addition, the adenosine triphosphate (ATP) content in cells and the activity of succinate dehydrogenase (SDH) both decreased when was exposed to different concentrations (1/4 MIC, 1/2 MIC, 1 MIC, 2 MIC) of luteolin for 1 h.

CONCLUSION

Luteolin showed distinct antibacterial activity against by multiple actions, which mainly include destroying the integrity of the cell wall and cell membrane, influencing the expression of proteins, inhibiting nucleic acid synthesis, and interfering with energy metabolism.