Guo Yuru, Liu Yan, Zhang Zehui, Chen Menghan, Zhang Dexian, Tian Chunlian, Liu Mingchun, Jiang Guotuo
Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, People's Republic of China.
Dalian Sanyi Animal Medicine Co., Ltd., Dalian, Liaoning, People's Republic of China.
Infect Drug Resist. 2020 Jun 10;13:1697-1711. doi: 10.2147/IDR.S253363. eCollection 2020.
This research aimed to investigate the antibacterial activity and potential mechanism of luteolin against .
The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of luteolin against various strains. The potential mechanism of action of luteolin was elucidated through testing and analysing the luteolin-induced alterations of in several aspects, including cell wall, cell membrane, protein expression, nucleic acid content, topoisomerase activity and energy metabolism.
The MIC values of luteolin against various isolates and ATCC19411 were 78 µg/mL. The increased cell membrane permeability, destruction of cell wall integrity and TEM images after exposure to luteolin showed that the cell wall and membrane were damaged. The content of total protein and nucleic acid in decreased significantly after treatment with luteolin (1/2 MIC) for 12, 24, and 36 h. Moreover, a hypochromic effect was observed in the absorption spectrum of luteolin when deoxyribonucleic acid (DNA) was added. In addition, after treatment with luteolin, a decrease in nicked or relaxed DNA content, which was catalysed by -isolated DNA topoisomerase, was observed. In addition, the adenosine triphosphate (ATP) content in cells and the activity of succinate dehydrogenase (SDH) both decreased when was exposed to different concentrations (1/4 MIC, 1/2 MIC, 1 MIC, 2 MIC) of luteolin for 1 h.
Luteolin showed distinct antibacterial activity against by multiple actions, which mainly include destroying the integrity of the cell wall and cell membrane, influencing the expression of proteins, inhibiting nucleic acid synthesis, and interfering with energy metabolism.
本研究旨在探讨木犀草素的抗菌活性及其潜在作用机制。
采用肉汤微量稀释法测定木犀草素对多种菌株的最低抑菌浓度（MIC）。通过测试和分析木犀草素在细胞壁、细胞膜、蛋白质表达、核酸含量、拓扑异构酶活性和能量代谢等多个方面引起的变化，阐明木犀草素的潜在作用机制。
木犀草素对各种分离菌株和ATCC19411的MIC值为78μg/mL。木犀草素作用后细胞膜通透性增加、细胞壁完整性破坏以及透射电镜图像显示细胞壁和细胞膜受损。用木犀草素（1/2 MIC）处理12、24和36小时后，菌株中总蛋白和核酸含量显著降低。此外，当加入脱氧核糖核酸（DNA）时，在木犀草素的吸收光谱中观察到减色效应。另外，用木犀草素处理后，观察到由分离的DNA拓扑异构酶催化的切口或松弛DNA含量降低。此外，当菌株暴露于不同浓度（1/4 MIC、1/2 MIC、1 MIC、2 MIC）的木犀草素1小时后，细胞中的三磷酸腺苷（ATP）含量和琥珀酸脱氢酶（SDH）活性均降低。
木犀草素通过多种作用对菌株表现出明显的抗菌活性，主要包括破坏细胞壁和细胞膜的完整性、影响蛋白质表达、抑制核酸合成以及干扰能量代谢。
