National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
Carbohydr Polym. 2018 Sep 1;195:378-386. doi: 10.1016/j.carbpol.2018.04.093. Epub 2018 Apr 30.
Exploring the long-term filler for minimally invasive plastic surgery has been widely concerned. In the present study, a series of injectable paste composed of hydroxyapatite (HAp) spherical particles and cross-linked sodium hyaluronate (cHA) solution were prepared. The physicochemical properties of cHA as a carrier of high content HAp microspheres (>50%) and as-obtained injectable HAp/cHA paste were studied. The cross-linking degree (DC), viscosity and molecular weight (Mw and Mn) of cHA increased with the increasing of the cross-linker dosage from 7.5 to 17.5 wt% under the certain conditions. HAp/cHA pastes were fabricated by homogeneously blending different sizes of HAp microspheres with cHA solution. The stability, rheological performance and push-out force of the pastes were studied, and the influence factors were discussed. The results indicated that moderate crosslinked cHA with 60% middle size HAp (HAp-M60/cHA-15.0) had appropriate comprehensive property. Finally, the in vitro cell culture approved the paste had no cytotoxicity. Although the biological performance of the pastes still need to be investigated, this preliminary study demonstrates that it is possible to carry high content HAp in cHA, expecting the better volumetric maintenance after long term implantation.
探索微创整形手术的长效填充物一直备受关注。本研究制备了一系列由羟基磷灰石（HAp）球形颗粒和交联透明质酸钠（cHA）溶液组成的可注射糊剂。研究了作为高含量 HAp 微球（>50%）载体的 cHA 的物理化学性质以及所获得的可注射 HAp/cHA 糊剂的性能。在一定条件下，交联剂用量从 7.5wt%增加到 17.5wt%时，cHA 的交联度（DC）、粘度和分子量（Mw 和 Mn）增加。通过将不同粒径的 HAp 微球均匀混合到 cHA 溶液中制备 HAp/cHA 糊剂。研究了糊剂的稳定性、流变性能和推出力，并探讨了影响因素。结果表明，具有 60%中等粒径 HAp（HAp-M60/cHA-15.0）的适度交联 cHA 具有适当的综合性能。最后，体外细胞培养证明该糊剂无细胞毒性。尽管糊剂的生物学性能仍需要进一步研究，但这项初步研究表明，在 cHA 中携带高含量 HAp 是可行的，期望在长期植入后具有更好的体积维持效果。
