Benameur Qada, Tali-Maamar Hassiba, Assaous Farida, Guettou Badia, Benklaouz Meki Boutaiba, Rahal Kheira, Ben-Mahdi Meriem-Hind
Faculty of Natural Sciences and Life, Abdelhamid Ibn Badis University, Mostaganem, Algeria.
Research Laboratory, Health and Animal Production, Higher National Veterinary School, Algiers, Algeria.
Vet World. 2018 Apr;11(4):469-473. doi: 10.14202/vetworld.2018.469-473. Epub 2018 Apr 12.
Multidrug-resistant (MDR) Enterobacteriaceae have frequently been reported, in both human and veterinary medicine, from different parts of the world as a consequence of antibiotic usage. However, there is a lack of published data regarding antimicrobial resistance in non- () Enterobacteriaceae from animals in Algeria. This study aimed to evaluate the frequency of resistance to antibiotics with a focus on quinolones and to investigate the presence of genes inEnterobacteriaceaeof poultry origin.
A total of 310 samples of poultry origin were collected from 2010 to 2014 from broiler and layer farms and hatcheries located in different geographic areas of Western Algeria (including Mostaganem, Oran, Mascara, Relizane, Chlef, Tiaret, and Tissemsilt). Antimicrobial susceptibility testing was performed using disc diffusion assay. Polymerase chain reaction and sequencing accomplished the characterization of genes (, , and ).
A total of 253 Enterobacteriaceaestrains were isolated in this study. These isolates exhibited high levels of resistance to quinolones and other families of antibiotics. All the strains isolated in this study were resistant to at least one antibiotic. Among them, 233 (92.09%) were considered MDR. Among the 18 randomly selected nalidixic acid (NA)-resistant Enterobacteriaceaeisolates, one and one were carrying . By contrast, and were not detected in this study.
This is the first report on the identification of the gene in isolated from animal source in Algeria. Further studies have to be conducted to determine the real prevalence of genes.
由于抗生素的使用，多药耐药（MDR）肠杆菌科细菌在人类和兽医学中频繁地在世界不同地区被报道。然而，关于阿尔及利亚动物来源的非（）肠杆菌科细菌的抗菌药物耐药性，缺乏已发表的数据。本研究旨在评估对包括喹诺酮类在内的抗生素的耐药频率，并调查家禽源肠杆菌科细菌中基因的存在情况。
2010年至2014年期间，从位于阿尔及利亚西部不同地理区域（包括穆斯塔加奈姆、奥兰、马斯卡拉、雷利赞、谢里夫、提亚雷特和提塞姆西勒）的肉鸡场、蛋鸡场和孵化场收集了总共310份家禽源样本。使用纸片扩散法进行抗菌药物敏感性测试。聚合酶链反应和测序完成了基因（、和）的特征分析。
本研究共分离出253株肠杆菌科菌株。这些分离株对喹诺酮类和其他抗生素家族表现出高水平的耐药性。本研究中分离的所有菌株至少对一种抗生素耐药。其中，233株（92.09%）被认为是多重耐药菌。在随机选择的18株对萘啶酸（NA）耐药的肠杆菌科分离株中，一株和一株携带。相比之下，本研究中未检测到和。
这是阿尔及利亚首次关于从动物源分离的中鉴定基因的报告。必须进行进一步研究以确定基因的实际流行情况。
