Li Yuxian, Guan Lili, Liu Xiuming, Liu Weican, Yang Jing, Zhang Xiaomei, Wang Fawei, Guo Yongxin, Li Haiyan, Li Xiaokun
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.
College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.
Exp Ther Med. 2018 Jun;15(6):4866-4874. doi: 10.3892/etm.2018.6003. Epub 2018 Mar 28.
Human rotavirus (HRV) is the primary cause of severe gastroenteritis in children. However, there is currently no protective virus for rotavirus available. In the present study, an HRVVP7-cholera toxin B subunit (CTB) fusion protein was expressed in . To determine the adjuvant effect of HRVVP7-CTB, HRVVP7 without CTB was expressed in the same manner. HRVVP7-CTB accounted for 0.39% of the total soluble protein (TSP) in the transgenic seeds and 52.65 µg/g of HRVVP7 protein was expressed in these seeds. Mice were immunized with TSP from the transformed seeds and produced serum immunoglobulin G (IgG) and mucosal IgA specifically directed against HRVVP7. Antibody titers were highest in mice orally immunized with the plant-expressed HRVVP7-CTB protein, whereas HRVVP7-CTB-specific IgG neutralized the rotavirus. Suckling pups born from dams immunized with the HRVVP7-CTB fusion protein were protected against challenge with virulent rotavirus. The results of the present study suggest that the HRVVP7-CTB fusion protein produced in may be a rotaviral-specific candidate subunit vaccine.
人类轮状病毒（HRV）是儿童严重肠胃炎的主要病因。然而，目前尚无针对轮状病毒的保护性疫苗。在本研究中，一种HRVVP7-霍乱毒素B亚基（CTB）融合蛋白在……中表达。为了确定HRVVP7-CTB的佐剂作用，以相同方式表达了不含CTB的HRVVP7。HRVVP7-CTB占转基因种子中总可溶性蛋白（TSP）的0.39%，这些种子中表达了52.65μg/g的HRVVP7蛋白。用转化种子中的TSP免疫小鼠，产生了特异性针对HRVVP7的血清免疫球蛋白G（IgG）和黏膜IgA。在用植物表达的HRVVP7-CTB蛋白口服免疫的小鼠中抗体滴度最高，而HRVVP7-CTB特异性IgG中和了轮状病毒。用HRVVP7-CTB融合蛋白免疫的母鼠所生的乳鼠受到保护，免受强毒轮状病毒的攻击。本研究结果表明，在……中产生的HRVVP7-CTB融合蛋白可能是一种轮状病毒特异性候选亚单位疫苗。
