Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow 226028, India.
Experimental & Public Health Lab, Department of Zoology, University of Lucknow, Lucknow 226007, India.
Int J Biol Macromol. 2020 Sep 1;158:159-179. doi: 10.1016/j.ijbiomac.2020.04.191. Epub 2020 Apr 29.
Human malaria is a pathogenic disease mainly caused by , which was responsible for about 405,000 deaths globally in the year 2018. To date, several vaccine candidates have been evaluated for prevention, which failed to produce optimal output at various preclinical/clinical stages. This study is based on designing of polypeptide vaccines (PVs) against human malaria that cover almost all stages of life-cycle of and for the same 5 genome derived predicted antigenic proteins (GDPAP) have been used. For the development of a multi-immune inducer, 15 PVs were initially designed using T-cell epitope ensemble, which covered >99% human population as well as linear B-cell epitopes with or without adjuvants. The immune simulation of PVs showed higher levels of T-cell and B-cell activities compared to positive and negative vaccine controls. Furthermore, cloning of PVs and codon optimization followed by enhanced expression within host system was also explored. Although, the study has sound theoretical and findings, the evaluation seems imperative to warrant the immunogenicity and safety of PVs towards management of infection in the future.
人类疟疾是一种致病性疾病，主要由疟原虫引起，2018 年全球约有 40.5 万人因此死亡。迄今为止，已经有几种疫苗候选物被评估用于预防疟疾，但在各种临床前/临床阶段都未能产生最佳效果。本研究基于设计针对人类疟疾的多肽疫苗（PVs），这些疫苗覆盖了疟原虫生命周期的几乎所有阶段，并且使用了相同的 5 种基于基因组预测的抗原蛋白（GDPAP）。为了开发多免疫诱导剂，最初使用 T 细胞表位集合设计了 15 种 PVs，这些表位集合覆盖了>99%的人类人群，以及具有或不具有佐剂的线性 B 细胞表位。与阳性和阴性疫苗对照相比，PVs 的免疫模拟显示出更高水平的 T 细胞和 B 细胞活性。此外，还探索了 PVs 的克隆和密码子优化以及在 宿主系统内的增强表达。尽管该研究具有合理的理论和 发现，但对 进行评估对于保证 PVs 的免疫原性和安全性以用于未来疟原虫感染的管理似乎是必要的。
