Chen Zaomei, Zhao Yue, He Ting, Chen Kejun, Jiang Li, Zhang Junhui, Wan Huayin, Li Rubing
Pharmaceutical college of Guangdong Pharmaceutical University, Guangzhou Guangdong, 510006, P.R.China;Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou Guangdong, 510010, P.R.China.
Guangzhou Electrospinning Biological Technology Co.Ltd., Guangzhou Guangdong, 510604, P.R.China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Aug 15;31(8):999-1005. doi: 10.7507/1002-1892.201703083.
Adopting poly- -lactic/glycolic acid (PLGA) and polyethylene glycol (PEG) as the material to fabricate PLGA/PEG electrospun polymer membrane by electrospinning technology. And to study its preventive effect on postoperative intraperitoneal adhesion of rat.
PLGA and PEG were mixed at the ratio of 19∶1( ), then dissolved in organic solvent. The PLGA/PEG electrospun polymer membrane was prepared by electrospinning technology, and then the gross observation and scanning electron microscope observation were taken. Fifty-four Sprague Dawley rats (weighing, 180-200 g), were randomly divided into 3 groups. The rats in control group ( =6) were left intact. The rats in model group ( =24) and PLGA/PEG group ( =24) were treated with the method of mechanical injury of the cecal serosa in order to establish the intraperitoneal adhesion models; then the PLGA/PEG electrospun polymer membrane was used to cover the wound in PLGA/PEG group, but was not in the model group. The intraperitoneal adhesion in PLGA/PEG group and model group were observed at 3 days, 1 week, 2 weeks, and 8 weeks after operation, and the adhesion degree was assessed according to the self-generated standard. The degradation of PLGA/PEG electrospun polymer membrane was also observed in PLGA/PEG group. At each time point, the rats were harvested for histological observation. All the above indexes were compared with the control group.
Using the electrospinning technology, PLGA/PEG electrospun polymer membrane was prepared successfully. PLGA/PEG electrospun polymer membrane was white and opaque, with soft texture. Scanning electron microscopy observation showed that PLGA/PEG electrospun polymer membrane was mainly composed of disorderly staggered fibers, with microporous structure. All rats survived to the end of the experiment. Gross observation showed that PLGA/PEG electrospun polymer membrane gradually degraded after implantation , and the adhesion degree in PLGA/PEG group was significantly lower than that in model group ( <0.05), but it had not yet reached to the level of the control group ( <0.05). Histological observation showed that the proliferation of cecal fibrous connective tissue was slower in PLGA/PEG group than in model group, and adhesion severity significantly decreased, only with a small amount of inflammatory cell infiltration. Nevertheless, it was not up to the level of the control group.
PLGA/PEG electrospun polymer membrane can effectively prevent postoperative intraperitoneal adhesion of rat, and has good biodegradability.
采用聚乳酸/乙醇酸共聚物（PLGA）和聚乙二醇（PEG）为材料，通过静电纺丝技术制备PLGA/PEG静电纺聚合物膜，并研究其对大鼠术后腹腔粘连的预防作用。
将PLGA与PEG按19∶1（ ）的比例混合，溶于有机溶剂。采用静电纺丝技术制备PLGA/PEG静电纺聚合物膜，然后进行大体观察和扫描电子显微镜观察。将54只Sprague Dawley大鼠（体重180 - 200 g）随机分为3组。对照组（ =6）大鼠不作处理。模型组（ =24）和PLGA/PEG组（ =24）采用盲肠浆膜机械损伤法建立腹腔粘连模型；然后在PLGA/PEG组用PLGA/PEG静电纺聚合物膜覆盖创面，模型组不覆盖。分别于术后3天、1周、2周和8周观察PLGA/PEG组和模型组腹腔粘连情况，并按自行制定的标准评估粘连程度。同时观察PLGA/PEG组PLGA/PEG静电纺聚合物膜的降解情况。在各时间点处死大鼠进行组织学观察。将上述各项指标与对照组比较。
利用静电纺丝技术成功制备了PLGA/PEG静电纺聚合物膜。PLGA/PEG静电纺聚合物膜呈白色不透明，质地柔软。扫描电子显微镜观察显示，PLGA/PEG静电纺聚合物膜主要由无序交错的纤维组成，具有微孔结构。所有大鼠均存活至实验结束。大体观察显示，PLGA/PEG静电纺聚合物膜植入后逐渐降解，PLGA/PEG组粘连程度明显低于模型组（ <0.05），但尚未达到对照组水平（ <0.05）。组织学观察显示，PLGA/PEG组盲肠纤维结缔组织增生较模型组缓慢，粘连严重程度明显降低，仅有少量炎性细胞浸润。但仍未达到对照组水平。
PLGA/PEG静电纺聚合物膜能有效预防大鼠术后腹腔粘连，且具有良好的生物降解性。
