Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Pharmaceutical Sciences Research Center, Shiraz University of Medical Science, Shiraz, Iran.
Biomed Res Int. 2021 Dec 28;2021:9977142. doi: 10.1155/2021/9977142. eCollection 2021.
Intra-abdominal adhesions following surgery are a challenging problem in surgical practice. This study fabricated different thermoplastic polyurethane (TPU) nanofibers with different average diameters using the electrospinning method. The conditions were evaluated by scanning electron microscopy (SEM), atomic force microscope (AFM), and Fourier transform infrared spectrometer (FTIR) analysis. A static tensile test was applied using a strength testing device to assess the mechanical properties of the electrospun scaffolds. By changing the effective electrospinning parameters, the best quality of nanofibers could be achieved with the lowest bead numbers. The electrospun nanofibers were evaluated using a rat cecal abrasion model. The macroscopic evaluation and the microscopic study, including the degree of adhesion and inflammation, were investigated after three and five weeks. The resultant electrospun TPU nanofibers had diameters ranging from about 200 to 1000 nm. The diameters and morphology of the nanofibers were significantly affected by the concentration of polymer. Uniform TPU nanofibers without beads could be prepared by electrospinning through reasonable control of the process concentration. These nanofibers' biodegradability and antibacterial properties were investigated by weight loss measurement and microdilution methods, respectively. The purpose of this study was to provide electrospun nanofibers having biodegradability and antibacterial properties that prevent any adhesions or inflammation after pelvic and abdominal surgeries. The experiments revealed that electrospun TPU nanofibers reduced the degree of abdominal adhesions. The histopathological study confirmed only a small extent of inflammatory cell infiltration in the 8% and 10% TPU. Conclusively, nanofibers containing 8% TPU significantly decreased the incidence and severity of postsurgical adhesions, and it is expected to be used in clinical applications in the future.
手术后的腹腔内粘连是外科实践中的一个难题。本研究采用静电纺丝法制备了不同平均直径的不同热塑性聚氨酯（TPU）纳米纤维。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）和傅里叶变换红外光谱（FTIR）分析评估条件。使用强度测试装置进行静态拉伸测试，评估电纺支架的机械性能。通过改变有效静电纺丝参数，可以获得珠粒数量最少的最佳质量纳米纤维。使用大鼠盲肠磨损模型评估电纺纳米纤维。在三到五周后，对宏观评估和包括粘连和炎症程度在内的微观研究进行了调查。所得的电纺 TPU 纳米纤维的直径范围约为 200 至 1000nm。纳米纤维的直径和形态明显受到聚合物浓度的影响。通过合理控制工艺浓度，可以制备出无珠的均匀 TPU 纳米纤维。通过重量损失测量和微稀释法分别研究了这些纳米纤维的生物降解性和抗菌性能。本研究的目的是提供具有生物降解性和抗菌性能的电纺纳米纤维，以防止骨盆和腹部手术后发生粘连或炎症。实验表明，电纺 TPU 纳米纤维可减少腹部粘连程度。组织病理学研究证实，8%和 10%TPU 的炎性细胞浸润程度仅很小。总之，含有 8%TPU 的纳米纤维可显著降低手术后粘连的发生率和严重程度，有望在未来的临床应用中使用。
