Seo Youngmin, Park Chanhwi, Son Jaewoo, Lee Kyungwoo, Hwang Jangsun, Jo Yeonho, Lee Dohyun, Khan Muhammad Saad, Chavan Sachin Ganpat, Choi Yonghyun, Kim Dasom, Gilad Assaf A, Choi Jonghoon
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology.
School of Integrative Engineering, Chung-Ang University.
J Vis Exp. 2018 May 10(135):57384. doi: 10.3791/57384.
In this study, multi-walled carbon nanotubes (MWCNTs) were treated with an aqueous sulfuric acid solution to form an oxygen-based functional group. Silver MWCNTs were prepared by the reductive deposition of silver from an aqueous solution of AgNO3 on the oxidized MWCNTs. Given the unique color of the CNTs, it was not possible to apply them to the minimum inhibitory concentration or mitochondrial toxicity assays to evaluate the toxicity and antibacterial properties, since they would interfere with the assays. The inhibition zone and minimum bactericidal concentration for the Ag-MWCNTs were measured and Live/Dead and Trypan Blue assays were used to measure the toxicity and antibacterial properties without interfering with the color of the CNTs.
在本研究中，多壁碳纳米管（MWCNTs）用硫酸水溶液处理以形成基于氧的官能团。通过将AgNO3水溶液中的银还原沉积在氧化的MWCNTs上制备银MWCNTs。鉴于碳纳米管独特的颜色，无法将它们应用于最低抑菌浓度或线粒体毒性测定以评估毒性和抗菌性能，因为它们会干扰测定。测量了Ag-MWCNTs的抑菌圈和最低杀菌浓度，并使用活/死和台盼蓝测定法来测量毒性和抗菌性能，而不会干扰碳纳米管的颜色。
