Thakur Vikas, Kumar Vijay, Kumar Sanjay, Singh Dharam
a Biotechnology Division, CSIR - Institute of Himalayan Bioresource Technology, Post Box No. 6, Palampur-176 061, Himachal Pradesh, India.
b Academy of Scientific and Innovative Research (AcSIR), CSIR - Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India.
Can J Microbiol. 2018 Nov;64(11):798-808. doi: 10.1139/cjm-2017-0754. Epub 2018 May 28.
The Pangi-Chamba Himalaya (PCH) region is very pristine, unique, and virgin niche for bioresource exploration. In the current study, for the first time, the bacterial diversity of this region was investigated for potential cellulose degraders. A total of 454 pure bacterial isolates were obtained from diverse sites in the PCH region, and 111 isolates were further selected for 16S rDNA characterization based on ARDRA grouping. The identified bacteria belonged to 28 genera representing four phyla: Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. Pseudomonas was most abundant genus, followed by Bacillus, Geobacillus, Arthrobacter, Paenibacillus, and Flavobacterium. In addition, six putative novel bacteria (based on 16S rDNA sequence similarity) and thermophiles from non-thermogenic sites were also reported for the first time. Screening for cellulose degradation ability on carboxymethyl cellulose plates revealed that 70.92% of bacteria were cellulolytic. The current study reports diverse bacterial genera (Arthrobacter, Paenibacillus, Chryseobacterium, Pedobacter, Streptomyces, Agromyces, Flavobacterium, and Pseudomonas) with high capacity for cellulose hydrolysis and cellulolytic functionality at wide pH and temperature not previously reported in the literature. Diverse bacterial genera with high cellulolytic activity in broad pH and temperature range provide opportunity to develop a bioprocess for efficient pretreatment of lignocellulosic biomass, which is currently being investigated.
潘吉 - 昌巴喜马拉雅（PCH）地区是生物资源勘探的非常原始、独特且未开发的生态位。在当前研究中，首次对该地区的细菌多样性进行了调查，以寻找潜在的纤维素降解菌。从PCH地区的不同地点共获得了454株纯细菌分离株，并基于ARDRA分组进一步选择了111株分离株进行16S rDNA鉴定。鉴定出的细菌属于代表四个门的28个属：厚壁菌门、变形菌门、放线菌门和拟杆菌门。假单胞菌是最丰富的属，其次是芽孢杆菌属、嗜热栖热菌属、节杆菌属、类芽孢杆菌属和黄杆菌属。此外，还首次报道了六种假定的新细菌（基于16S rDNA序列相似性）以及来自非产热地点的嗜热菌。在羧甲基纤维素平板上筛选纤维素降解能力表明，70.92%的细菌具有纤维素分解能力。当前研究报道了多种具有高纤维素水解能力且在宽pH和温度下具有纤维素分解功能的细菌属（节杆菌属、类芽孢杆菌属、金黄杆菌属、土壤杆菌属、链霉菌属、土壤微杆菌属、黄杆菌属和假单胞菌属），这些在文献中此前未见报道。在宽pH和温度范围内具有高纤维素分解活性的多种细菌属为开发一种用于木质纤维素生物质高效预处理的生物工艺提供了机会，目前正在对此进行研究。
