Chamorro C A, de Paz P, Villar J M, Fernández M
Department of Anatomy and Embryology, Faculty of Veterinary, Leon, Spain.
Histol Histopathol. 1987 Jan;2(1):67-72.
Head mesoderm cells from chick embryos at different stages of development were dissociated and cultured on plastic coverslips. In all cultures several cellular aggregates were described by means of scanning electron microscopy. Isolated cells present filopodia and lamellipodia. However, when mesoderm cells make contact with one another the filopodial and lamellipodial activity in the contact cellular edge disappear. Thus, the cells into cellular clusters do not present projections. The clusters were circular and bidimensional in character. The scanning electron microscopic observations showed that it is the type 1 variant of "contact inhibition of locomotion" which occurs. By means of these mechanisms the bidimensional aggregates are formed and cellular overlapping is not present. Since the behaviour of the mesoderm cells "in vitro" in some way could be comparable to their behaviour "in situ", the results here observed are discussed in relation to the conduct of mesoderm cells "in vivo".
将来自不同发育阶段鸡胚的头部中胚层细胞解离，并培养在塑料盖玻片上。在所有培养物中，通过扫描电子显微镜观察到了几个细胞聚集体。分离的细胞呈现丝状伪足和片状伪足。然而，当中胚层细胞相互接触时，接触细胞边缘的丝状伪足和片状伪足活动消失。因此，细胞聚集体中的细胞没有突起。这些聚集体呈圆形且为二维结构。扫描电子显微镜观察表明，发生的是“运动接触抑制”的1型变体。通过这些机制形成了二维聚集体，且不存在细胞重叠。由于中胚层细胞“体外”行为在某种程度上可能与其“原位”行为具有可比性，因此本文观察到的结果结合中胚层细胞“体内”行为进行了讨论。
