ICMR Virus Unit, ID & BG Hospital, GB-4, First Floor, 57 Dr Suresh C Banerjee Road, Beliaghata, Kolkata-700010, West Bengal, India.
J Ethnopharmacol. 2013 Aug 26;149(1):335-43. doi: 10.1016/j.jep.2013.06.045. Epub 2013 Jul 6.
Different parts of Indian ethnomedicinal plant Shorea robusta is traditionally used for several ailments including wounds and burn by different tribal groups, since ages. Here we have validated, for the first time, the effectiveness and the possible mechanism of action of young leaf extracts of Shorea robusta, used by two distinct tribes of India, and its isolated compounds as a topical formulation in three wound models in rats.
Bioactivity-guided study of the active extract resulted in the isolation of two known compounds. The prepared ointment containing extracts (2.5 and 5%, w/w), fractions (5% w/w) and isolated compounds (0.25% w/w) were evaluated on excision, incision and dead space wound models in rats by the rate of wound closure, period of epithelialisation, tensile strength, granulation tissue weight, hydroxyproline content and histopathology.
The animals treated with the extracts and fractions (5%) showed significant reduction in wound area 96.55 and 96.41% with faster epithelialisation (17.50 and 17.86), while the isolated compounds bergenin and ursolic acid heal the wound faster, but complete epithelialisation with 100% wound contraction was evident with 5% povidone-iodine group on 18th post-wounding day. Moreover, the tensile strength of incision wound, granuloma tissue weight, and hydroxyproline content was significantly increased in both the extract and compound(s) treated animals. Furthermore, the tissue histology of animals treated with the isolated compound(s) showed complete epithelialisation with increased collagenation, similar to povidone-iodine group.
Thus, our results validated the traditional use of Shorea robusta young leaves in wound management.
不同的印度药用植物印度娑罗双树的部分传统上用于多种疾病，包括不同部落群体的伤口和烧伤，自古至今。在这里，我们首次验证了印度两个不同部落使用的印度娑罗双树幼叶提取物及其分离化合物作为局部制剂在三种大鼠伤口模型中的有效性和可能的作用机制。
活性提取物的基于生物活性的研究导致两种已知化合物的分离。含有提取物（2.5%和 5%，w/w）、馏分（5%w/w）和分离化合物（0.25%w/w）的软膏制剂在大鼠的切除、切开和死腔伤口模型中进行了评估，评估指标包括伤口闭合率、上皮化时间、拉伸强度、肉芽组织重量、羟脯氨酸含量和组织病理学。
用提取物和馏分（5%）处理的动物显示出显著减少的伤口面积，分别为 96.55%和 96.41%，同时具有更快的上皮化（17.50%和 17.86%），而分离化合物 Bergenin 和 Ursolic Acid 则更快地愈合伤口，但在第 18 天的创伤后，用 5%聚维酮碘组可以实现 100%的伤口收缩和完全上皮化。此外，在提取物和化合物处理的动物中，切口伤口的拉伸强度、肉芽组织重量和羟脯氨酸含量显著增加。此外，用分离化合物治疗的动物的组织学研究显示出完全的上皮化，胶原蛋白化增加，类似于聚维酮碘组。
因此，我们的结果验证了印度娑罗双树幼叶在伤口管理中的传统用途。
