Al-Shammari Nour M, Shafshak Sanaa M, Ali Mohamed S
Division of Periodontics, Department of Preventive Dental Science, Riyadh Elm University, Riyadh, Kingdom of Saudi Arabia, e-mail:
Division of Periodontics, Department of Preventive Dental Science, Riyadh Elm University, Riyadh, Kingdom of Saudi Arabia.
J Contemp Dent Pract. 2018 May 1;19(5):527-534.
The aim of this study is to evaluate the effect of the subgingival application of 0.8% hyaluronic acid (HA) gel (GENGIGEL®) as an adjunct to scaling and root planing (SRP) on clinical parameters and expression of human beta defensin-2 (hBD-2) in patients with moderate to severe chronic periodontitis.
In this randomized, split mouth design study, 24 participants with moderate to severe chronic periodontitis were evaluated after full mouth SRP. In the test sites 1 mL of 0.8% hyaluronan gel was applied subgingivally after SRP at baseline and 1 week post therapy. Plaque index (PI), gingival index (GI), papillary bleeding index (PBI), periodontal probing depth (PPD), and clinical attachment loss (CAL) were recorded and gingival crevicular fluid (GCF) samples were collected at baseline, after 6 and 12 weeks. Expression of human beta defensin-2 (hBD-2) was analyzed by enzyme-linked immunosorbent assay.
At baseline, there were no statistical differences between test and control sites in all clinical parameters and hBD-2 expression. An improvement of PI, GI, PBI, PPD, and CAL was observed at 6 and 12 weeks (p < 0.05) in both groups. Clinically, it was noticed that all indices except CAL had more statistically significant reduction in test sites than control sites at 6 and 12 weeks. The hBD-2 levels were significantly higher in the test sites than in the control sites at 6 and 12 weeks.
The local application of 0.8% hyaluronan gel with SRP have a positive effect on periodontal health of moderate to severe chronic periodontitis patients after 6 and 12 weeks.
Subgingival application of 0.8% HA gel following SRP has shown anti-inflammatory effect and has a beneficial effect on clinical parameters in moderate to severe chronic periodontitis patients.
本研究旨在评估在中度至重度慢性牙周炎患者中，龈下应用0.8%透明质酸（HA）凝胶（GENGIGEL®）作为龈上洁治和根面平整（SRP）辅助治疗手段对临床参数及人β-防御素-2（hBD-2）表达的影响。
在这项随机、双侧对照设计研究中，24名中度至重度慢性牙周炎患者在全口SRP后接受评估。在试验部位，SRP后基线及治疗后1周龈下应用1 mL 0.8%透明质酸钠凝胶。记录菌斑指数（PI）、牙龈指数（GI）、龈乳头出血指数（PBI）、牙周探诊深度（PPD）和临床附着丧失（CAL），并在基线、6周和12周后收集龈沟液（GCF）样本。采用酶联免疫吸附测定法分析人β-防御素-2（hBD-2）的表达。
基线时，试验组和对照组在所有临床参数及hBD-2表达方面均无统计学差异。两组在6周和12周时PI、GI、PBI、PPD和CAL均有改善（p < 0.05）。临床上，在6周和12周时，除CAL外，试验组所有指标的改善在统计学上均比对照组更显著。6周和12周时，试验组的hBD-2水平显著高于对照组。
龈下应用0.8%透明质酸钠凝胶联合SRP在6周和12周后对中度至重度慢性牙周炎患者的牙周健康有积极影响。
SRP后龈下应用0.8% HA凝胶显示出抗炎作用，对中度至重度慢性牙周炎患者的临床参数有有益影响。
