Roth H P, Kirchgessner M
Institut für Ernährungsphysiologie, Technischen Universität München, Fed. Rep. of Germany.
J Trace Elem Electrolytes Health Dis. 1988 Jun;2(2):73-8.
To demonstrate the effect of zinc and/or calcium deficiency on calmodulin concentration and also the antagonistic behavior of zinc and calcium in metabolism, four groups of eight rats each were provided the following diets in equal amounts: group I, the basic diet with 63 ppm zinc and 0.88% calcium; group II, the calcium-deficient diet (0.0043% calcium); group III, the zinc-deficient diet (1.1 ppm zinc); and group IV, the combined zinc/calcium-deficient diet. The zinc-deficient diet significantly reduced the zinc concentration in serum (72%), femur (65%), pancrease (34%), muscle (23%), testis (11%) and brain (7%), and elevated the calcium concentration in testis (19%) and brain (24%), but did not affect calcium concentration in serum, pancreas and muscle. Likewise, calcium deficiency reduced the calcium concentration in serum (56%), femur (40%), pancreas (16%), muscle (43%), testis (19%) and brain (24%), and elevated the zinc concentration in femur (22%), although the zinc concentration in serum, pancreas, muscle and testis remained unaffected. Combined zinc and calcium deficiency elevated both the zinc as well as the calcium concentration in serum, femur and muscle, as compared to zinc or calcium deficiency alone. The alkaline phosphatase activity was reduced by 52% during zinc deficiency and was elevated by a factor of 2.5 during calcium deficiency. The calmodulin concentrations in muscle, testis and brain, as determined by radioimmunoassay, were elevated during both zinc as well as calcium deficiency, although this elevation could not be confirmed in every case.
为了证明锌和/或钙缺乏对钙调蛋白浓度的影响，以及锌和钙在代谢中的拮抗作用，将四组大鼠（每组八只）等量给予以下饮食：第一组，含63 ppm锌和0.88%钙的基础饮食；第二组，缺钙饮食（0.0043%钙）；第三组，缺锌饮食（1.1 ppm锌）；第四组，锌/钙联合缺乏饮食。缺锌饮食显著降低了血清（72%）、股骨（65%）、胰腺（34%）、肌肉（23%）、睾丸（11%）和大脑（7%）中的锌浓度，并提高了睾丸（19%）和大脑（24%）中的钙浓度，但不影响血清、胰腺和肌肉中的钙浓度。同样，钙缺乏降低了血清（56%）、股骨（40%）、胰腺（16%）、肌肉（43%）、睾丸（19%）和大脑（24%）中的钙浓度，并提高了股骨（22%）中的锌浓度，尽管血清、胰腺、肌肉和睾丸中的锌浓度未受影响。与单独缺锌或缺钙相比，锌和钙联合缺乏提高了血清、股骨和肌肉中的锌以及钙浓度。碱性磷酸酶活性在缺锌时降低了52%，在缺钙时升高了2.5倍。通过放射免疫测定法测定，肌肉、睾丸和大脑中的钙调蛋白浓度在缺锌和缺钙时均升高，尽管并非在每种情况下都能证实这种升高。
