Law J S, McBride S A, Graham S, Nelson N R, Slotnick B M, Henkin R I
Center for Molecular Nutrition and Sensory Disorders, Washington, DC 20007.
Life Sci. 1987 Dec 14;41(24):2597-606. doi: 10.1016/0024-3205(87)90273-6.
Two groups of male Sprague-Dawley rats, one fed zinc-deficient diet, ad libitum, the other, pair-fed with the same diet, but given supplemental zinc in the drinking water (8 mg Zn++/ml) were studied. After ten weeks of diet, rats were exsanguinated and zinc and calmodulin concentrations in brain and testis were measured. Mean zinc concentration in testis was significantly decreased in rats fed zinc-deficient diet without supplemental Zn++, but mean zinc concentration in brain was not different. Similarly, mean calmodulin concentration in testis was decreased in rats fed zinc-deficient diet without supplemental Zn++ whereas mean calmodulin concentration in brain was not different. Distribution studies of zinc and calmodulin showed that both zinc and calmodulin were released more freely into soluble fractions of testis in rats fed zinc-deficient diet without supplemental Zn++. These results indicate, for the first time in in vivo studies, that zinc influences the calmodulin content of testis.
研究了两组雄性斯普拉格-道利大鼠，一组随意喂食缺锌饮食，另一组与前者喂食相同饮食，但在饮用水中补充锌（8毫克锌离子/毫升）。饮食十周后，处死大鼠并测量大脑和睾丸中的锌和钙调蛋白浓度。喂食缺锌饮食且未补充锌离子的大鼠睾丸中的平均锌浓度显著降低，但大脑中的平均锌浓度没有差异。同样，喂食缺锌饮食且未补充锌离子的大鼠睾丸中的平均钙调蛋白浓度降低，而大脑中的平均钙调蛋白浓度没有差异。锌和钙调蛋白的分布研究表明，在喂食缺锌饮食且未补充锌离子的大鼠中，锌和钙调蛋白都更自由地释放到睾丸的可溶性部分中。这些结果在体内研究中首次表明，锌会影响睾丸中的钙调蛋白含量。
