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1
Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.针对劳氏肉瘤病毒src基因产物的高度特异性抗体识别出一种新的pp60v-src和pp60c-src分子群体。
J Cell Biol. 1985 Feb;100(2):409-17. doi: 10.1083/jcb.100.2.409.
2
Highly specific antibody to Rous sarcoma virus src gene product recognizes nuclear and nucleolar antigens in human cells.针对劳氏肉瘤病毒src基因产物的高度特异性抗体可识别人类细胞中的核抗原和核仁抗原。
J Virol. 1995 Mar;69(3):1699-713. doi: 10.1128/JVI.69.3.1699-1713.1995.
3
Phosphotyrosine-containing 120,000-dalton protein coimmunoprecipitated with pp60v-src from Rous sarcoma virus-transformed mammalian cells.来自劳氏肉瘤病毒转化的哺乳动物细胞的含磷酸酪氨酸的120,000道尔顿蛋白与pp60v-src共免疫沉淀。
Virology. 1986 May;151(1):86-99. doi: 10.1016/0042-6822(86)90106-6.
4
pp60v-src tyrosine kinase is expressed and active in sarcoma-free avian embryos microinjected with Rous sarcoma virus.pp60v-src酪氨酸激酶在注射了劳氏肉瘤病毒的无肉瘤禽胚胎中表达并具有活性。
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7587-91. doi: 10.1073/pnas.85.20.7587.
5
Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.禽肉瘤病毒转化细胞的免疫荧光：src基因产物的定位
Cell. 1979 Jan;16(1):11-24. doi: 10.1016/0092-8674(79)90183-1.
6
Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src).劳氏肉瘤病毒转化蛋白（pp60v-src）及其正常细胞同源物（pp60c-src）中酪氨酸磷酸化位点的鉴定
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6013-7. doi: 10.1073/pnas.78.10.6013.
7
The use of Rous sarcoma virus transformation mutants with differing tyrosine kinase activities to study the relationships between vinculin phosphorylation, pp60v-src location and adhesion plaque integrity.利用具有不同酪氨酸激酶活性的劳氏肉瘤病毒转化突变体来研究纽蛋白磷酸化、pp60v-src定位与黏着斑完整性之间的关系。
Exp Cell Res. 1986 Jul;165(1):216-28. doi: 10.1016/0014-4827(86)90546-x.
8
Evidence that a phosphotyrosine-containing 120,000 Da protein from Rous sarcoma virus-infected cells is phosphorylated by pp60v-src.来自劳氏肉瘤病毒感染细胞的一种含磷酸酪氨酸的120,000道尔顿蛋白质被pp60v-src磷酸化的证据。
Oncogene Res. 1989;4(3):185-94.
9
Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.与劳氏肉瘤病毒src基因同源的细胞基因的结构与序列以及产生转化病毒的机制。
Cell. 1983 Mar;32(3):881-90. doi: 10.1016/0092-8674(83)90073-9.
10
Identification of transformation-specific proteins synthesized in cryptovirogenic mammalian cells.鉴定在隐病毒发生性哺乳动物细胞中合成的转化特异性蛋白。
Folia Biol (Praha). 1985;31(2):152-60.

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A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer.一种新型核Src和p300信号轴控制胰腺癌的迁移和侵袭行为。
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Generation of H9 T-cells stably expressing a membrane-bound form of the cytoplasmic tail of the Env-glycoprotein: lack of transcomplementation of defective HIV-1 virions encoding C-terminally truncated Env.稳定表达Env糖蛋白胞质尾膜结合形式的H9 T细胞的产生：缺乏对编码C末端截短Env的缺陷型HIV-1病毒体的反式互补作用。
Retrovirology. 2006 May 16;3:27. doi: 10.1186/1742-4690-3-27.
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Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain.Lyn蛋白通过高尔基体小窝蛋白的运输涉及激酶结构域中αE和αI螺旋上的带电残基。
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6
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Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):8-10. doi: 10.1073/pnas.98.1.8.
7
Kinetic analysis of human immunodeficiency virus type 1 assembly reveals the presence of sequential intermediates.1型人类免疫缺陷病毒组装的动力学分析揭示了连续中间体的存在。
J Virol. 2000 Jul;74(13):5845-55. doi: 10.1128/jvi.74.13.5845-5855.2000.
8
Incorporation of wild-type and C-terminally truncated human epidermal growth factor receptor into human immunodeficiency virus-like particles: insight into the processes governing glycoprotein incorporation into retroviral particles.野生型和C末端截短的人表皮生长因子受体掺入人免疫缺陷病毒样颗粒：深入了解逆转录病毒颗粒中糖蛋白掺入的调控过程。
J Virol. 1999 Nov;73(11):9294-302. doi: 10.1128/JVI.73.11.9294-9302.1999.
9
Human immunodeficiency virus type 1 protease triggers a myristoyl switch that modulates membrane binding of Pr55(gag) and p17MA.1型人类免疫缺陷病毒蛋白酶引发豆蔻酰开关，调节Pr55(gag)和p17MA的膜结合。
J Virol. 1999 Mar;73(3):1902-8. doi: 10.1128/JVI.73.3.1902-1908.1999.
10
Differential membrane binding of the human immunodeficiency virus type 1 matrix protein.人类免疫缺陷病毒1型基质蛋白的差异性膜结合
J Virol. 1996 Dec;70(12):8540-8. doi: 10.1128/JVI.70.12.8540-8548.1996.

本文引用的文献

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Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells.培养的正常细胞和恶性细胞中糖部分的亚细胞区室化
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Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.识别禽肉瘤病毒转化蛋白的单克隆抗体的分离
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9
Interaction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex.劳氏肉瘤病毒转化蛋白与两种细胞磷蛋白之间的相互作用：该复合物的周转和分布分析。
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The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid.劳氏肉瘤病毒、哈维氏肉瘤病毒和阿贝尔逊病毒的转化蛋白含有紧密结合的脂质。
Cell. 1982 Dec;31(2 Pt 1):465-74. doi: 10.1016/0092-8674(82)90139-8.

针对劳氏肉瘤病毒src基因产物的高度特异性抗体识别出一种新的pp60v-src和pp60c-src分子群体。

Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.

作者信息

Resh M D, Erikson R L

出版信息

J Cell Biol. 1985 Feb;100(2):409-17. doi: 10.1083/jcb.100.2.409.

DOI:10.1083/jcb.100.2.409
PMID:2981886
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2113423/
Abstract

Antiserum to the Rous sarcoma virus (RSV)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed in Escherichia coli. alpha p60 serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (TBR) sera. When RSV-transformed cell lysates were preadsorbed with TBR serum, the remaining lysate contained additional pp60v-src, which was recognized only by reimmunoprecipitation with alpha p60 serum and not by TBR serum. In subcellular fractions of RSV-infected chicken embryo fibroblasts (RSV-CEFs) and field vole cells probed with TBR serum, the majority of the pp60v-src was associated with the plasma membrane-enriched P100 fraction. However, alpha p60 serum revealed equal distribution of pp60v-src and its kinase activity between the P1 (nuclear) and P100 fractions. The same results were obtained for pp60c-src in uninfected CEFs. On discontinuous sucrose gradients nearly 50% of the P1-pp60v-src sedimented with nuclei, in fractions where no plasma membrane was detected. Indirect immunofluorescence microscopy of RSV-CEFs with alpha p60 serum revealed a distinct pattern of perinuclear fluorescence, in addition to staining at the cell periphery. Thus the use of a highly specific antibody reveals that enzymatically active pp60v-src and pp60c-src molecules are present in other intracellular structures, probably juxtareticular nuclear membranes, in addition to the plasma membrane in normal, uninfected, and wild-type RSV-infected cells.

摘要

用在大肠杆菌中表达的p60免疫家兔，制备了抗劳斯肉瘤病毒（RSV）转化蛋白pp60v-src的抗血清。α p60血清比荷瘤兔（TBR）血清能更定量地免疫沉淀pp60v-src。当RSV转化的细胞裂解物用TBR血清预吸附时，剩余的裂解物中含有额外的pp60v-src，该蛋白仅能被α p60血清再免疫沉淀识别，而不能被TBR血清识别。在用TBR血清检测的RSV感染的鸡胚成纤维细胞（RSV-CEF）和野田鼠细胞的亚细胞组分中，大部分pp60v-src与富含质膜的P100组分相关。然而，α p60血清显示pp60v-src及其激酶活性在P1（核）和P100组分之间分布均匀。未感染的CEF中的pp60c-src也得到了相同的结果。在不连续蔗糖梯度上，近50%的P1-pp60v-src与细胞核一起沉淀，在未检测到质膜的组分中。用α p60血清对RSV-CEF进行间接免疫荧光显微镜检查，除了细胞周边染色外，还显示出明显的核周荧光模式。因此，使用高度特异性抗体表明，在正常、未感染和野生型RSV感染的细胞中，除了质膜外，具有酶活性的pp60v-src和pp60c-src分子还存在于其他细胞内结构中，可能是近网状核膜。