关于大肠杆菌recA蛋白促使互补DNA链复性的机制
On the mechanism of renaturation of complementary DNA strands by the recA protein of Escherichia coli.
作者信息
Bryant F R, Lehman I R
出版信息
Proc Natl Acad Sci U S A. 1985 Jan;82(2):297-301. doi: 10.1073/pnas.82.2.297.
Abstract
The renaturation of complementary DNA strands by the recA protein of Escherichia coli has been found to exhibit the following features. (i) Optimal renaturation occurs at recA protein levels below that required to saturate the DNA strands; saturating amounts of recA protein significantly reduce the rate of reaction. (ii) The reaction proceeds in the absence of a nucleotide cofactor but is markedly stimulated by ATP in the presence of 10 mM Mg2+. A similar stimulation occurs in the absence of ATP when the Mg2+ concentration is increased from 10 mM to 30-40 mM. (iii) Both the ATP-stimulated and the Mg2+-stimulated reactions follow apparent first-order kinetics. These results, taken together with the known effects of ATP and Mg2+ on the state of aggregation of recA protein, suggest that the association of recA monomers may play an important role in recA protein-promoted DNA renaturation.
摘要
已发现大肠杆菌的RecA蛋白促使互补DNA链复性具有以下特点。（i）最佳复性发生在RecA蛋白水平低于使DNA链饱和所需的水平时；RecA蛋白达到饱和量会显著降低反应速率。（ii）反应在没有核苷酸辅因子的情况下进行，但在存在10 mM Mg2+时，ATP会显著刺激该反应。当Mg2+浓度从10 mM增加到30 - 40 mM时，在没有ATP的情况下也会发生类似的刺激。（iii）ATP刺激的反应和Mg2+刺激的反应均遵循明显的一级动力学。这些结果，连同ATP和Mg2+对RecA蛋白聚集状态的已知影响，表明RecA单体的缔合可能在RecA蛋白促进的DNA复性中起重要作用。
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