Gerbling K P, Steup M, Latzko E
Eur J Biochem. 1985 Feb 15;147(1):207-15. doi: 10.1111/j.1432-1033.1985.tb08738.x.
Extracts of Synechococcus leopoliensis (Anacystis nidulans) contain two forms of D-fructose-1,6-bisphosphatase (EC 3.1.3.11) previously designated as forms A and B [Gerbling, K.-P., Steup, M., and Latzko, E. (1984) Arch. Microbiol. 137, 109-114]. Form B, which probably represents the major part of the total extractable fructose-1,6-bisphosphatase activity, has been purified to apparent homogeneity. Gel filtration, non-denaturing polyacrylamide gel electrophoresis, and cross-linking with bis(sulfosuccinimidyl)suberate revealed that the fructose-1,6-bisphosphatase B exists in either a dimeric or in a tetrameric subform, depending upon the absence or presence of fructose-1,6-bisphosphate and Mg2+. The dimer--tetramer interconversion was readily reversible. The results provide evidence for a two-step activation of fructose-1,6-bisphosphatase B involving the reduction of the dimeric subform and the subsequent substrate-dependent conversion of the reduced dimer to a reduced tetramer, which is the only catalytically active state. In contrast to form B, no substrate-dependent interconversion was detected with form A from S. leopoliensis.
波兰集胞藻（集胞藻属）提取物含有两种形式的D-果糖-1,6-二磷酸酶（EC 3.1.3.11），以前分别命名为A和B型[格布林，K.-P.，施图普，M.，和拉茨科，E.（1984年）《微生物学档案》137卷，第109 - 114页]。B型可能代表了总可提取果糖-1,6-二磷酸酶活性的主要部分，已被纯化至表观均一。凝胶过滤、非变性聚丙烯酰胺凝胶电泳以及与双（磺基琥珀酰亚胺基）辛二酸酯交联显示，果糖-1,6-二磷酸酶B以二聚体或四聚体亚形式存在，这取决于果糖-1,6-二磷酸和Mg2 +的有无。二聚体 - 四聚体的相互转化很容易逆转。结果为果糖-1,6-二磷酸酶B的两步激活提供了证据，这涉及二聚体亚形式的还原以及随后还原二聚体向还原四聚体的底物依赖性转化，还原四聚体是唯一具有催化活性的状态。与B型不同，未检测到来自波兰集胞藻的A型存在底物依赖性相互转化。
