Gupta M, Coffino P
J Biol Chem. 1985 Mar 10;260(5):2941-4.
cDNA containing the full coding region of mouse ornithine decarboxylase was isolated. The complete nucleotide sequence of the cDNA was determined by the dideoxy method, and the amino acid sequence of ornithine decarboxylase was thereby deduced. The protein contains 461 amino acids and has a molecular weight of 51,172. The isoelectric point is predicted from the deduced amino acid sequence to be 5.1. On the basis of its amino acid sequence, the protein is predicted to be comprised predominantly of alternating domains of alpha-helix and beta-sheet.
分离得到了包含小鼠鸟氨酸脱羧酶完整编码区的cDNA。通过双脱氧法确定了该cDNA的完整核苷酸序列，从而推导出鸟氨酸脱羧酶的氨基酸序列。该蛋白质含有461个氨基酸，分子量为51,172。根据推导的氨基酸序列预测其等电点为5.1。根据其氨基酸序列预测，该蛋白质主要由α-螺旋和β-折叠交替结构域组成。
