Hickok N J, Seppänen P J, Gunsalus G L, Jänne O A
DNA. 1987 Jun;6(3):179-87. doi: 10.1089/dna.1987.6.179.
A complementary DNA (cDNA) encoding ornithine decarboxylase was isolated from a human liver cDNA library, and the nucleotide sequence coding for the entire enzyme was determined. The 1825-nucleotide-long cDNA contained an open reading frame of 1383 nucleotides, 87 nucleotides 5' from the first methionine codon, 346 nucleotides in the 3'-noncoding region, and a poly(A) tail of nine bases. Primer extension studies indicated that the 5'-noncoding region of the human ornithine decarboxylase mRNA was 335 nucleotides long. The amino acid sequence deduced from the open reading frame for a 461-residue polypeptide predicts a molecular weight of 51.156 for the human enzyme and has about 90% homology with the amino acid sequence of the murine ornithine decarboxylase (44 differences among the 461 amino acids). The nucleotide sequences of the human and murine ornithine decarboxylase mRNAs share an 85% homology, even in their 3'-noncoding regions. In contrast to rodent tissues with two ornithine decarboxylase mRNAs, normal human tissues appear to express only a single mRNA species with a molecular size of 2.25 kb. Southern blotting of human leukocyte DNA from 20 individuals indicated that the ornithine decarboxylase gene belongs to a multigene family in man and showed restriction fragment length polymorphism when cleaved with Pst I, but not when cleaved with Pvu II, Msp I, Hinc II, or Bam HI.
从人肝脏cDNA文库中分离出编码鸟氨酸脱羧酶的互补DNA（cDNA），并测定了编码整个酶的核苷酸序列。这个1825个核苷酸长的cDNA包含一个1383个核苷酸的开放阅读框，第一个甲硫氨酸密码子上游87个核苷酸，3'非编码区346个核苷酸，以及一个9个碱基的聚腺苷酸尾。引物延伸研究表明，人鸟氨酸脱羧酶mRNA的5'非编码区长335个核苷酸。从461个氨基酸残基的多肽开放阅读框推导的氨基酸序列预测人酶的分子量为51156，与小鼠鸟氨酸脱羧酶的氨基酸序列有大约90%的同源性（461个氨基酸中有44个不同）。人和小鼠鸟氨酸脱羧酶mRNA的核苷酸序列即使在其3'非编码区也有85%的同源性。与具有两种鸟氨酸脱羧酶mRNA的啮齿动物组织不同，正常人组织似乎只表达一种分子大小为2.25kb的mRNA。对20个人的人白细胞DNA进行Southern印迹分析表明，鸟氨酸脱羧酶基因属于人类的一个多基因家族，用Pst I切割时显示限制性片段长度多态性，但用Pvu II、Msp I、Hinc II或Bam HI切割时则不显示。
