Murtaugh M P, Kapoor C L, Denlinger D L
J Exp Zool. 1985 Mar;233(3):413-23. doi: 10.1002/jez.1402330310.
The male accessory reproductive gland (ARG) of the house cricket, Acheta domesticus (L.), contains an exceedingly high concentration of cyclic GMP, about 1,000 pmol/mg protein. Immunofluorescent localization and radioimmunoassay measurements show that cyclic GMP is concentrated in a small number of tubules. It accumulates in the tubule lumina where it is protected from degradation by phosphodiesterases. Cyclic GMP is secreted by the ARG and is incorporated into spermatophores. Over 80% of spermatophore cyclic GMP is found in the handle-capillary tube, a thin conduit through which sperm pass during transfer to the female. The concentration of cyclic GMP in the insemination fluid is about 20 microM but does not appear to be specifically associated with the sperm. Cyclic GMP enters the female spermatheca during insemination but disappears rapidly. Physiological effects of cyclic GMP on sperm were not observed nor was an effect of cyclic GMP observed on egg laying by mated females. Cyclic AMP was localized on sperm flagella in the spermatophore and in the spermatheca. These studies indicate that cyclic nucleotides have important roles in insect reproduction and that the house cricket is a good model for elucidating these functions.
家蟋蟀（Acheta domesticus (L.)）的雄性附属生殖腺（ARG）含有极高浓度的环鸟苷酸（cGMP），约为1000皮摩尔/毫克蛋白质。免疫荧光定位和放射免疫测定显示，cGMP集中在少数小管中。它在小管腔中积累，在那里它受到磷酸二酯酶的保护而不被降解。cGMP由ARG分泌并被整合到精荚中。超过80%的精荚cGMP存在于柄-毛细管中，精子在转移到雌性体内时通过的一条细管道。授精液中环鸟苷酸的浓度约为20微摩尔，但似乎与精子没有特异性关联。cGMP在授精过程中进入雌性受精囊，但很快消失。未观察到cGMP对精子的生理作用，也未观察到cGMP对已交配雌性产卵的影响。环磷酸腺苷（cAMP）定位在精荚和受精囊中的精子鞭毛上。这些研究表明，环核苷酸在昆虫繁殖中具有重要作用，家蟋蟀是阐明这些功能的良好模型。
