用于组胺能细胞定位的抗L-组氨酸脱羧酶单克隆抗体。

Monoclonal antibody against L-histidine decarboxylase for localization of histaminergic cells.

作者信息

Pollard H, Pachot I, Schwartz J C

出版信息

Neurosci Lett. 1985 Feb 28;54(1):53-8. doi: 10.1016/s0304-3940(85)80117-8.

Abstract

The immunochemical and immunohistochemical properties of a monoclonal antibody (Mab HI 113-12) developed in mice against a partially purified preparation of rat gastric L-histidine decarboxylase (HD) were studied. The Mab recognised the HD activity from the antigen and from crude tissue extracts with a high histamine (HA)-synthesizing capacity (stomach, hypothalamus, striatum, mastocytoma). In contrast, neither a bacterial HD nor other decarboxylases (glutamic and DOPA decarboxylases) were immunoprecipitated. In preliminary immunohistochemical studies, staining of the cytoplasm of mastocytoma as well as of hypothalamic neurons, particularly magnocellular ones in the mamillary region, were observed. The latter presumably correspond to the cells of origin of a long ascending histaminergic pathway.

摘要

研究了在小鼠中制备的一种针对大鼠胃L-组氨酸脱羧酶（HD）部分纯化制剂的单克隆抗体（Mab HI 113-12）的免疫化学和免疫组织化学特性。该单克隆抗体识别来自抗原以及具有高组胺（HA）合成能力的粗组织提取物（胃、下丘脑、纹状体、肥大细胞瘤）中的HD活性。相比之下，细菌HD和其他脱羧酶（谷氨酸脱羧酶和多巴脱羧酶）均未被免疫沉淀。在初步免疫组织化学研究中，观察到肥大细胞瘤以及下丘脑神经元的细胞质染色，特别是乳头体区域的大细胞神经元。后者可能对应于一条长的上行组胺能通路的起源细胞。