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通过对应分析检测总大肠菌群和粪大肠菌群计数中的假阳性

Detection of false-positives among total and fecal coliform counts by factorial analysis of correspondence.

作者信息

Joncas M, Michaud S, Carmichael J P, Lavoie M C

出版信息

Appl Environ Microbiol. 1985 Jan;49(1):229-31. doi: 10.1128/aem.49.1.229-231.1985.

Abstract

Application of an analysis of correspondence to the biochemical characteristics of total and fecal coliforms isolated in the Ivory Coast permitted us to separate two small clusters of isolates different from the main clusters, which included isolates from human and animal feces. The isolates grouped in the small clusters were from water samples. An analysis of the biochemical characteristics which permitted the segregation of the "water-specific" isolates from the main clusters indicates that water-specific total coliforms were citrate positive, indole negative, and amygdaline positive. Water-specific fecal coliforms were either citrate positive, indole negative, amygdaline positive, and inositol negative or indole negative, amygdaline positive, and inositol positive. Any isolates not fitting the above patterns could be considered of fecal origin. If this observation is confirmed under temperate climates and for a greater number of isolates, these simple tests could be used to confirm the fecal origin of coliforms.

摘要

通过将对应分析应用于在象牙海岸分离出的总大肠菌群和粪大肠菌群的生化特性,我们能够区分出两小群与主要菌群不同的分离株,主要菌群包括来自人类和动物粪便的分离株。归入小群的分离株来自水样。对生化特性的分析使得能够将“水特异性”分离株与主要菌群区分开来,结果表明水特异性总大肠菌群柠檬酸盐阳性、吲哚阴性、扁桃苷阳性。水特异性粪大肠菌群要么柠檬酸盐阳性、吲哚阴性、扁桃苷阳性、肌醇阴性,要么吲哚阴性、扁桃苷阳性、肌醇阳性。任何不符合上述模式的分离株都可被认为源自粪便。如果在温带气候下以及针对更多分离株证实了这一观察结果,那么这些简单测试可用于确认大肠菌群的粪便来源。

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