N，N'-二环己基碳二亚胺对线粒体细胞色素bc1复合物质子转运影响的热力学和稳态动力学研究
Thermodynamic and steady-state-kinetic investigation of the effect of NN'-dicyclohexylcarbodi-imide on H+ translocation by the mitochondrial cytochrome bc1 complex.
作者信息
Brand M D, Al-Shawi M K, Brown G C, Price B D
出版信息
Biochem J. 1985 Jan 15;225(2):407-11. doi: 10.1042/bj2250407.
Abstract
Steady-state kinetic measurements showed that NN'-dicyclohexylcarbodi-imide decreased the observed H+/2e ratio of H+ transport by mitochondria respiring on succinate, acting mainly at the cytochrome bc1 complex. Thermodynamic assessment of the H+/2e ratio by measuring the force ratio across the bc1 complex showed that the inhibitor did not affect H+ translocation. Possible explanations of this disagreement between methods are examined; we conclude that the inhibitor does not alter the mechanistic stoichiometry of H+ pumping by the bc1 complex.
摘要
稳态动力学测量表明，N，N'-二环己基碳二亚胺降低了琥珀酸呼吸的线粒体中观察到的H⁺/2e⁺的H⁺转运比率，主要作用于细胞色素bc1复合体。通过测量bc1复合体两侧的力比来对H⁺/2e⁺比率进行热力学评估，结果表明该抑制剂不影响H⁺的转运。本文研究了两种方法之间这种差异的可能解释；我们得出结论，该抑制剂不会改变bc1复合体泵送H⁺的机制化学计量。
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引用本文的文献
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Biochem J. 1986 Aug 1;237(3):927-30. doi: 10.1042/bj2370927.
本文引用的文献
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J Biol Chem. 1980 Nov 25;255(22):10721-30.
2
N,N'-Dicyclohexylcarbodiimide inhibits monovalent cation influx but not cation/proton exchange in heart mitochondria.N，N'-二环己基碳二亚胺抑制心脏线粒体中的单价阳离子内流，但不抑制阳离子/质子交换。
J Biol Chem. 1980 Jan 25;255(2):408-12.
3
Dicyclohexylcarbodiimide modification of bovine heart mitochondrial transhydrogenase.二环己基碳二亚胺对牛心线粒体转氢酶的修饰作用
J Biol Chem. 1981 Sep 10;256(17):8963-9.
4
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