通过线粒体细胞色素bc1复合物对电子通量的热力学控制。
Thermodynamic control of electron flux through mitochondrial cytochrome bc1 complex.
作者信息
Brown G C, Brand M D
出版信息
Biochem J. 1985 Jan 15;225(2):399-405. doi: 10.1042/bj2250399.
Abstract
The redox states of exogenously added ubiquinone-2 and cytochrome c, and the protonmotive force (delta p) of rat liver mitochondria were measured as the respiration rate was titrated with the uncoupler carbonyl cyanide p-trifluoromethoxyphenyl-hydrazone. The force ratio delta Eh/delta p across the bc1 complex was close to 1:1 in State 4, indicating an H+/e- stoichiometry of 1:1 for the cytochrome bc1 complex, excluding protons moved by pool ubiquinone. Assuming a constant stoichiometry the rate of electron transport increased linearly with the disequilibrium (delta Eh - delta p) across the complex.
摘要
在用解偶联剂羰基氰化物对三氟甲氧基苯腙滴定呼吸速率时，测定了外源性添加的辅酶Q-2和细胞色素c的氧化还原状态以及大鼠肝脏线粒体的质子动力（Δp）。在状态4中，bc1复合物两侧的力比ΔEh/Δp接近1:1，这表明细胞色素bc1复合物的H⁺/e⁻化学计量比为1:1，不包括泛醌池移动的质子。假设化学计量恒定，电子传递速率随复合物两侧的不平衡（ΔEh - Δp）呈线性增加。
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