Stewart D I, Crawford N
Biochem J. 1985 Feb 1;225(3):807-14. doi: 10.1042/bj2250807.
In the analysis of highly purified surface membrane from both resting and phagocytosing neutrophils an increase in the surface membrane associated actin has been demonstrated. This change at the cell periphery is associated with a coincident increase in the F-actin content of the cells following stimulation of the cells by exposure to opsonized Oil Red O droplets. The actin which is newly associated with the surface membrane of the phagocytosing cells was more susceptible to removal by detergent than the membrane-associated actin in resting cells and it was also noted that the F-actin associated with phagosomes was readily disrupted by detergent. A redistribution of the surface membrane glycoprotein 5'-nucleotidase was observed during phagocytosis, but no change in distribution of a 125I-labelled Lens culinaris lectin was observed during the entire phagocytic process.
在对静息和吞噬状态的中性粒细胞高度纯化的表面膜进行分析时，已证明与表面膜相关的肌动蛋白有所增加。细胞外周的这种变化与经调理素化的油红O液滴刺激细胞后细胞中F-肌动蛋白含量的同时增加有关。与吞噬细胞表面膜新结合的肌动蛋白比静息细胞中与膜相关的肌动蛋白更易被去污剂去除，并且还注意到与吞噬体相关的F-肌动蛋白很容易被去污剂破坏。在吞噬过程中观察到表面膜糖蛋白5'-核苷酸酶的重新分布，但在整个吞噬过程中未观察到125I标记的菜豆凝集素分布的变化。
