Chua K Y, Pankhurst C E, Macdonald P E, Hopcroft D H, Jarvis B D, Scott D B
J Bacteriol. 1985 Apr;162(1):335-43. doi: 10.1128/jb.162.1.335-343.1985.
Rhizobium loti NZP2037 and NZP2213, each cured of its single large indigenous plasmid, formed effective nodules on Lotus spp., suggesting that the symbiotic genes are carried on the chromosome of these strains. By using pSUP1011 as a vector for introducing transposon Tn5 into R. loti NZP2037, symbiotic mutants blocked in hair curling (Hac), nodule initiation (Noi), bacterial release (Bar), and nitrogen fixation (Nif/Cof) on Lotus pedunculatus were isolated. Cosmids complementing the Hac, Noi, and Bar mutants were isolated from a pLAFR1 gene library of NZP2037 DNA by in planta complementation and found to contain EcoRI fragments of identical sizes to those into which Tn5 had inserted in the mutants. The cosmids that complemented the mutants of these phenotypic classes did not share common fragments, nor did cosmids that complemented four mutants within the Noi class, suggesting that these symbiotically important regions are not tightly linked on the R. loti chromosome.
百脉根根瘤菌NZP2037和NZP2213，各自去除了其单个大型内源质粒后，能在百脉根属植物上形成有效的根瘤，这表明共生基因位于这些菌株的染色体上。通过使用pSUP1011作为载体将转座子Tn5导入百脉根根瘤菌NZP2037，分离出了在百脉根上出现卷毛（Hac）、根瘤起始（Noi）、细菌释放（Bar）和固氮（Nif/Cof）受阻的共生突变体。通过植物体内互补从NZP2037 DNA的pLAFR1基因文库中分离出了互补Hac、Noi和Bar突变体的黏粒，发现其含有与Tn5插入突变体中的片段大小相同的EcoRI片段。互补这些表型类别的突变体的黏粒没有共同片段，互补Noi类别内四个突变体的黏粒也没有共同片段，这表明这些共生重要区域在百脉根根瘤菌染色体上并非紧密连锁。
