a Division of Toxicology, College of Pharmacy , Sungkyunkwan University , Suwon , South Korea.
b McLaughlin Centre for Population Health Risk Assessment , University of Ottawa , Ottawa , ON , Canada.
J Toxicol Environ Health A. 2018;81(16):748-760. doi: 10.1080/15287394.2018.1473262. Epub 2018 May 29.
The endocrine disrupting actions of di(2-ethylhexyl) phthalate (DEHP) on testicular functions are postulated to involve excess free radical generation. Thus the aim of this study was to examine the ability of antioxidant vitamins C and E to prevent DEHP-induced testicular disruption in male Sprague-Dawley (SD) rats. SD male rats were administered DEHP alone or DEHP with vitamin C and/or vitamin E for 30 days. DEHP alone increased the levels of testosterone (T) and reduced estradiol (E) concentrations. Supplementation with antioxidant vitamins diminished or restored serum T levels noted in DEHP-treated rats to control values. In contrast vitamins C and E increased E levels to control in rats administered DEHP. Antioxidants significantly improved the decreased testicular levels of reduced glutathione and activity of superoxide dismutase compared to DEHP-treatment alone. Co-treatment of vitamins C and E also markedly improved the reduced epididymal sperm head counts and elevated levels of malondialdehyde (MDA) or 8-hydroxydeoxyguanosine (8-OHdG) induced by DEHP treatment. These results support the concept that the adverse actions of DEHP may be related to increased free radical generation while co-treatment with vitamins C and E significantly blocked the actions of DEHP on male testicular functions.
邻苯二甲酸二（2-乙基己基）酯（DEHP）对睾丸功能的内分泌干扰作用被认为涉及过多的自由基生成。因此，本研究旨在研究抗氧化维生素 C 和 E 是否能够预防 DEHP 诱导的雄性 Sprague-Dawley（SD）大鼠睾丸损伤。雄性 SD 大鼠单独给予 DEHP 或 DEHP 与维生素 C 和/或维生素 E 共处理 30 天。单独给予 DEHP 增加了睾酮（T）的水平，降低了雌二醇（E）的浓度。抗氧化维生素的补充减少或恢复了 DEHP 处理大鼠的血清 T 水平至对照值。相比之下，维生素 C 和 E 使给予 DEHP 的大鼠的 E 水平升高至对照值。与单独 DEHP 处理相比，抗氧化剂显著改善了睾丸组织中还原型谷胱甘肽水平和超氧化物歧化酶活性的降低。维生素 C 和 E 的共同处理还显著改善了 DEHP 处理引起的附睾精子头部计数减少和丙二醛（MDA）或 8-羟基脱氧鸟苷（8-OHdG）水平升高。这些结果支持 DEHP 的不良作用可能与自由基生成增加有关的观点，而维生素 C 和 E 的共同处理显著阻断了 DEHP 对雄性睾丸功能的作用。
