Mohsenzadeh Mehdi, Salehi-Abargouei Amin, Tabibnejad Nasim, Karimi-Zarchi Mojgan, Khalili Mohammad Ali
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Gerash Al-Zahra Fertility Center, Gerash University of Medical Sciences, Gerash, Iran.
Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Department of Nutrition, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Cryobiology. 2018 Aug;83:84-89. doi: 10.1016/j.cryobiol.2018.05.003. Epub 2018 May 26.
Combination of in vitro maturation (IVM) and cryopreservation offers new opportunities for women with contraindication in ovarian stimulation, and females who desire to postpone the childbearing due to different problems. There are still controversies regarding IVM procedure and its impact on oocytes fertilization capability. This systematic review and meta-analysis were conducted to evaluate the impact of vitrification on human oocyte maturation rate during IVM procedure. In this review, we searched Medline, Embase, Scopus and ISI web of science to identify English-language studies. The last search was implemented on 3 February 2018. The original articles which assessed maturation rate after vitrification of MI or GV oocytes were included. Animal trials and the studies that performed cryopreservation using slow-freeze method were excluded. Bias and quality assessments were performed. 2476 articles were screened primarily. After duplication removing and the application of inclusion and exclusion criteria, 14 studies included for the analysis. All studies compared maturation rate between the oocytes that were vitrified at the GV or MI stage before maturation and oocytes which were matured in vitro without vitrification. Meta-analysis showed that oocyte vitrification at GV stage had a significant negative impact on maturation rate (RR = 0.76, 95% CI: 0.66-0.88); I = 85.2%; P = 0.000). Finally, based on our results, oocyte vitrification decreases the maturation rate by 24%.
体外成熟（IVM）与冷冻保存相结合为卵巢刺激有禁忌证的女性以及因各种问题希望推迟生育的女性提供了新的机会。关于IVM程序及其对卵母细胞受精能力的影响仍存在争议。本系统评价和荟萃分析旨在评估玻璃化对IVM程序中人类卵母细胞成熟率的影响。在本评价中，我们检索了Medline、Embase、Scopus和ISI科学网以识别英文研究。最后一次检索于2018年2月3日进行。纳入评估MI或GV卵母细胞玻璃化后成熟率的原始文章。排除动物试验和使用慢速冷冻法进行冷冻保存的研究。进行了偏倚和质量评估。初步筛选了2476篇文章。在去除重复项并应用纳入和排除标准后，纳入14项研究进行分析。所有研究均比较了在GV或MI阶段成熟前玻璃化的卵母细胞与未玻璃化体外成熟的卵母细胞之间的成熟率。荟萃分析表明，GV期卵母细胞玻璃化对成熟率有显著负面影响（RR = 0.76，95%CI：0.66 - 0.88；I = 85.2%；P = 0.000）。最后，根据我们的结果，卵母细胞玻璃化使成熟率降低了24%。
