Youm H S, Choi J R, Oh D, Rho Y H
Center for Reproductive Medicine, Eroom Women's Clinic, Busan, Republic of Korea.
Cryo Letters. 2017 Nov/Dec;38(6):455-462.
T he most commonly used methods for the cryopreservation of oocytes and embryos are vitrification and slow freezing.
The aim of this study was to to investigate whether there are differences in survival, in vitro maturation (IVM), and fertilization rates between cryopreserved immature oocytes, especially germinal vesicle (GV)-stage human oocytes, following vitrification and slow freezing.
A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases. A total of three studies were included in the Bayesian meta-analysis.
There was no difference in survival rates between vitrification and slow freezing. Additionally, there was no difference in IVM rates and fertilization rates between vitrification and slow freezing.
The superiority of vitrification over slow freezing for cryopreservation of GV-stage human oocytes remains unclear. Additional studies on cytoarchitecture and modification of the cryopreservation protocol are essential to achieve strong conclusions.
卵母细胞和胚胎冷冻保存最常用的方法是玻璃化冷冻和慢速冷冻。
本研究旨在探讨玻璃化冷冻和慢速冷冻后，冷冻保存的未成熟卵母细胞，尤其是生发泡（GV）期人类卵母细胞在存活率、体外成熟（IVM）率和受精率方面是否存在差异。
使用MEDLINE、Cochrane对照试验中央注册库和Embase数据库进行文献检索。三项研究纳入了贝叶斯荟萃分析。
玻璃化冷冻和慢速冷冻的存活率没有差异。此外，玻璃化冷冻和慢速冷冻的IVM率和受精率也没有差异。
玻璃化冷冻在GV期人类卵母细胞冷冻保存方面优于慢速冷冻仍不明确。关于细胞结构和冷冻保存方案修改的进一步研究对于得出有力结论至关重要。
