Cho Seungchan, Kim Dongjun, Lee Yongjun, Kil Eui-Joon, Cho Mun-Ju, Byun Sung-June, Cho Won Kyong, Lee Sukchan
Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon 16419, Korea.
Animal Biotechnology Division, National Institute of Animal Science (NIAS), Rural Development Administration (RDA), 1500, Kongjwipatjwi-ro, Iseomyeon, Wanju 55365, Korea.
Genes (Basel). 2018 May 29;9(6):276. doi: 10.3390/genes9060276.
Probiotics are well known for their beneficial effects for animals, including humans and livestock. Here, we tested the probiotic activity of expressing 3D8 scFv, a nucleic acid-hydrolyzing mini-antibody, in mice intestine. A total of 18 fecal samples derived from three different conditions at two different time points were subjected to high-throughput 16S ribosomal RNA (rRNA) metagenomic analyses. Bioinformatic analyses identified an average of 290 operational taxonomic units. After administration of , populations of the probiotics , , and increased, whereas the population of harmful bacteria such as species decreased. Furthermore, continuous administration of resulted in emerging as the dominant probiotic after competition with other existing probiotics. Expression of 3D8 scFv protein specifically increased the population of , which is another probiotic. In summary, our results showed that expressing 3D8 scFv protein enhanced probiotic activity in mice intestine with no observable side effects. Thus, the system developed in this study may be a good tool for the expression of recombinant protein using probiotics.
益生菌因其对包括人类和牲畜在内的动物的有益作用而闻名。在此，我们测试了在小鼠肠道中表达3D8单链抗体片段（scFv，一种核酸水解微型抗体）的益生菌活性。对来自两个不同时间点三种不同条件下的总共18份粪便样本进行了高通量16S核糖体RNA（rRNA）宏基因组分析。生物信息学分析平均鉴定出290个操作分类单元。施用[具体物质]后，益生菌[具体种类1]、[具体种类2]和[具体种类3]的数量增加，而诸如[有害细菌种类]等有害细菌的数量减少。此外，持续施用[具体物质]导致[具体益生菌种类]在与其他现有益生菌竞争后成为主要的益生菌。3D8 scFv蛋白的表达特异性地增加了另一种益生菌[具体益生菌种类]的数量。总之，我们的结果表明，表达3D8 scFv蛋白可增强小鼠肠道中的益生菌活性，且无明显副作用。因此，本研究开发的系统可能是利用益生菌表达重组蛋白的良好工具。
