Department of Biomedical Engineering, Rochester Institute of Technology, Rochester, NY, USA.
Lab Chip. 2018 Jun 12;18(12):1671-1689. doi: 10.1039/c7lc01248a.
Porous membranes enable the partitioning of cellular microenvironments in vitro, while still allowing physical and biochemical crosstalk between cells, a feature that is often necessary for recapitulating physiological functions. This article provides an overview of the different membranes used in tissue barrier and cellular co-culture models with a focus on experimental design and control of these systems. Specifically, we discuss how the structural, mechanical, chemical, and even the optical and transport properties of different membranes bestow specific advantages and disadvantages through the context of physiological relevance. This review also explores how membrane pore properties affect perfusion and solute permeability by developing an analytical framework to guide the design and use of tissue barrier or co-culture models. Ultimately, this review offers insight into the important aspects one must consider when using porous membranes in tissue barrier and lab-on-a-chip applications.
多孔膜能够在体外分隔细胞微环境，同时仍允许细胞间的物理和生化串扰，这是再现生理功能的常用手段。本文概述了组织屏障和细胞共培养模型中使用的不同膜，重点介绍了这些系统的实验设计和控制。具体来说，我们讨论了不同膜的结构、力学、化学，甚至光学和传输特性如何通过与生理相关性的结合，赋予特定的优缺点。本文还通过开发分析框架来探讨膜孔特性如何通过影响灌注和溶质渗透性，从而指导组织屏障或共培养模型的设计和使用。最终，本文深入探讨了在组织屏障和芯片实验室应用中使用多孔膜时必须考虑的重要方面。
