Yoo Jin, Jung Youngmee, Char Kookheon, Jang Yeongseon
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; School of Electrical and Electronic Engineering, YU-KIST Institute, Yonsei University, Seoul 03722, Republic of Korea.
Trends Biotechnol. 2023 Feb;41(2):214-227. doi: 10.1016/j.tibtech.2022.07.014. Epub 2022 Aug 24.
Porous membranes play a critical role in in vitro heterogeneous cell coculture systems because they recapitulate the in vivo microenvironment to mediate physical and biochemical crosstalk between cells. While the conventionally available Transwell® system has been widely used for heterogeneous cell coculture, there are drawbacks to precise control over cell-cell interactions and separation for implantation. The size and numbers of the pores and the thickness of the porous membranes are crucial in determining the efficiency of paracrine signaling and direct junctions between cocultured cells, and significantly impact on the performance of heterogeneous cell cultures. These opportunities and challenges have motivated the design of advanced coculture platforms through improvement of the structural and functional properties of porous membranes.
多孔膜在体外异质细胞共培养系统中起着关键作用，因为它们能够重现体内微环境，介导细胞间的物理和生化相互作用。虽然传统的Transwell®系统已广泛用于异质细胞共培养，但在精确控制细胞间相互作用以及用于植入的细胞分离方面存在缺点。孔的大小、数量以及多孔膜的厚度对于确定旁分泌信号传导的效率和共培养细胞之间的直接连接至关重要，并显著影响异质细胞培养的性能。这些机遇和挑战促使人们通过改进多孔膜的结构和功能特性来设计先进的共培养平台。
