Haag M, Gevers W, Böhmer R G
Mol Cell Biochem. 1985 Mar;66(2):111-6. doi: 10.1007/BF00220778.
The interaction of noradrenaline, various cation chelators and calcium on Na+, K+-ATPase from rat cerebral cortex plasma membranes was studied. It was shown that chelation of inhibitory cations by EGTA, EDTA and dipyridyl activated Na+, K+-ATPase to the same extent as noradrenaline but at higher concentrations; increasing concentrations of EGTA depressed the activation by noradrenaline; calcium in the form of a calcium-EGTA buffer depressed Na+, K+-ATPase at physiological concentrations; the inhibition of Na+, K+-ATPase by calcium is dependent on the magnesium concentration in the assay and the inhibition by calcium was partially reversed by noradrenaline.
研究了去甲肾上腺素、各种阳离子螯合剂和钙对大鼠大脑皮质质膜Na + ，K + -ATP酶的相互作用。结果表明，EGTA、EDTA和联吡啶对抑制性阳离子的螯合作用与去甲肾上腺素一样能激活Na + ，K + -ATP酶，但所需浓度更高；EGTA浓度增加会抑制去甲肾上腺素的激活作用；钙-EGTA缓冲液形式的钙在生理浓度下会抑制Na + ，K + -ATP酶；钙对Na + ，K + -ATP酶的抑制作用取决于测定中镁的浓度，而去甲肾上腺素可部分逆转钙的抑制作用。
