Adám-Vizi V, Ordögh M, Horváth I, Somogyi J, Vizi E S
J Neural Transm. 1980;47(1):53-60. doi: 10.1007/BF01256639.
The effect of l-noradrenaline and vanadate on the activity of Na+K+-activated ATPase was studied on synaptosomal brain cortex preparation. Using neutron activation analysis it was found that the rat cerebral cortex synaptosomal preparation contains 0.16 microM vanadium. The concentration of vanadium needed to reduce enzyme activity by 50% proved to be 2 x 10(-6) M. Evidence has been provided that the increase by noradrenaline of enzyme activity in synaptosomal preparation depends on the presence of an inhibitory contaminant in commercial ATP preparations. In homogenate, however, noradrenaline was able to enhance enzyme activity even when vanadium-free ATP was used. This fact indicates that noradrenaline removes the inhibitory effect of cytoplasmic factor thereby stimulating enzyme activity.
在脑皮质突触体标本上研究了L-去甲肾上腺素和钒酸盐对钠钾激活的ATP酶活性的影响。通过中子活化分析发现，大鼠大脑皮质突触体标本含有0.16微摩尔钒。结果表明，使酶活性降低50%所需的钒浓度为2×10⁻⁶ M。已有证据表明，去甲肾上腺素使突触体标本中酶活性增加取决于市售ATP制剂中存在的一种抑制性污染物。然而，在匀浆中，即使使用无钒ATP，去甲肾上腺素也能增强酶活性。这一事实表明，去甲肾上腺素消除了细胞质因子的抑制作用，从而刺激了酶活性。
