光控基因表达：光笼鸟嘌呤和核糖开关的作用。
Optochemical control of gene expression by photocaged guanine and riboswitches.
机构信息
Nucleic Acid Chemistry and Engineering Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904 0495, Japan.
出版信息
Chem Commun (Camb). 2018 Jun 12;54(48):6181-6183. doi: 10.1039/c8cc02290a.
PMID:29845982
Abstract
Optical control of biomolecules via engineered proteins or photoactive small molecules has had a profound impact on biology. However, optochemical tools to control RNA functions in living cells are relatively limited. We synthesized a photoactivatable (photocaged) guanine to modulate gene expression under riboswitch control in both mammalian cells and Escherichia coli by light.
摘要
通过工程化蛋白质或光活性小分子对生物分子进行光学控制，对生物学产生了深远的影响。然而，用于控制活细胞中 RNA 功能的光化学工具相对有限。我们合成了一种光活化（光笼）鸟嘌呤，通过光在哺乳动物细胞和大肠杆菌中受核酶控制来调节基因表达。
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