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[Determination of kinase activity by using an enzyme electrode].

作者信息

Kurtinaĭtene B S, Kulis Iu Iu

出版信息

Prikl Biokhim Mikrobiol. 1985 Jan-Feb;21(1):141-4.

PMID:2984658
Abstract

A method for determining the enzymatic activity of hexokinases, acetate kinase and pyruvate kinase using an enzyme electrode was developed. The assay time is 2-3 min. The lower limit of the activity determining is 0,054 U/ml. The proposed method was applied to investigation of pyruvate kinase and acetate kinase reactivation under the action of mercaptoethanol.

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