Kurtinaĭtene B S, Kulis Iu Iu
Prikl Biokhim Mikrobiol. 1985 Jan-Feb;21(1):141-4.
A method for determining the enzymatic activity of hexokinases, acetate kinase and pyruvate kinase using an enzyme electrode was developed. The assay time is 2-3 min. The lower limit of the activity determining is 0,054 U/ml. The proposed method was applied to investigation of pyruvate kinase and acetate kinase reactivation under the action of mercaptoethanol.
开发了一种使用酶电极测定己糖激酶、乙酸激酶和丙酮酸激酶酶活性的方法。测定时间为2 - 3分钟。活性测定的下限为0.054 U/ml。所提出的方法被应用于研究巯基乙醇作用下丙酮酸激酶和乙酸激酶的再激活。