[一种测定丙酮酸激酶活性的方法]
[A method of determining pyruvate kinase activity].
作者信息
Korkach V I
出版信息
Ukr Biokhim Zh (1978). 1986 Mar-Apr;58(2):70-2.
PMID:3705205
Abstract
A method is suggested for determining the activity of pyruvate kinase (ATP: pyruvate-phosphotransferase). The method is based on evaluation of the amount of ATP formed in the course of phosphoenolpyruvic-to-pyruvic acid transformation in the presence of ADP and magnesium ions. The ATP amount in the reaction medium is determined by paper electrophoresis with subsequent spectrophotometry.
摘要
提出了一种测定丙酮酸激酶（ATP：丙酮酸磷酸转移酶）活性的方法。该方法基于在二磷酸腺苷（ADP）和镁离子存在的情况下，评估磷酸烯醇丙酮酸转化为丙酮酸过程中形成的三磷酸腺苷（ATP）的量。反应介质中的ATP量通过纸电泳和随后的分光光度法测定。
Zotero 插件