Rodriguez-Furlán Cecilia, Rubilar-Hernández Carlos, Norambuena Lorena
Faculty of Sciences, Department of Biology, Plant Molecular Biology Centre, Universidad de Chile, Ñuñoa, Chile.
Methods Mol Biol. 2018;1795:189-201. doi: 10.1007/978-1-4939-7874-8_16.
Chemical genomics has proven to be a useful and successful approach to study complex systems where conventional genetics fails to render feasible results. High-throughput phenotype screenings in model organisms have identified a large collection of powerful and selective bioactive chemicals. Nevertheless, applying chemical high-throughput screening to crops still represents a big challenge for researchers. Fortunately, a circumvent approach could be taken by means of translational research. In this case, searching bioactive chemicals in a much handy model organism would be the starting point for discovering compounds with activity in relevant plants for improving a desirable trait. In this chapter, we describe strategies that have been proven to successfully translate chemical biology and genetics from unicellular yeast to Arabidopsis thaliana and finally to crops.
化学基因组学已被证明是一种有用且成功的方法，用于研究传统遗传学无法得出可行结果的复杂系统。在模式生物中进行的高通量表型筛选已鉴定出大量强效且具有选择性的生物活性化学物质。然而，将化学高通量筛选应用于作物对研究人员来说仍然是一个巨大的挑战。幸运的是，可以通过转化研究采取一种规避方法。在这种情况下，在一种更便于操作的模式生物中搜索生物活性化学物质将是发现对相关植物具有活性以改善理想性状的化合物的起点。在本章中，我们描述了已被证明能成功地将化学生物学和遗传学从单细胞酵母转化到拟南芥，最终转化到作物的策略。
