Xu Yan-Jun, Elimban Vijayan, Bhullar Sukhwinder K, Dhalla Naranjan S
Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Department of Physiology and Pathophysiology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R2H 2A6, Canada.
Can J Physiol Pharmacol. 2018 Oct;96(10):1017-1021. doi: 10.1139/cjpp-2018-0160. Epub 2018 May 30.
The effects of CO water-bath therapy on the hind limb of diabetic animals with or without peripheral ischemia were examined. Diabetes was induced in rats by administering streptozotocin (65 mg·kg), and the animals were then divided into 3 groups. After 4 weeks, peripheral ischemia was induced by ligation of the femoral artery for 2 weeks in 2 groups (diabetic ischemic) of diabetic rats, whereas the femoral artery was not occluded in the third group (diabetic). All these animals were subjected to water-bath therapy (with or without CO mixing; 20 min·day for 5 days·week) for a period of 4 weeks. Both peak and mean flows, unlike minimal flow, in diabetic ischemic limbs were increased about a twofold by CO water-bath treatment. Morphological examination of hind limb tissue sections revealed about a twofold increase in the small artery count in diabetic ischemic animals upon CO water-bath treatment. These results indicate that CO water-bath therapy augments the blood flow and development of angiogenesis in the skeletal muscle of diabetic ischemic animals and thus may be of some benefit for the treatment of peripheral arterial disease in diabetes.
研究了一氧化碳水浴疗法对伴有或不伴有外周缺血的糖尿病动物后肢的影响。通过给予链脲佐菌素（65mg·kg）诱导大鼠患糖尿病，然后将动物分为3组。4周后，对2组糖尿病大鼠（糖尿病缺血组）结扎股动脉2周以诱导外周缺血，而第3组（糖尿病组）的股动脉未闭塞。所有这些动物均接受为期4周的水浴疗法（有或没有混合一氧化碳；每天20分钟，每周5天）。与最小血流量不同，一氧化碳水浴治疗使糖尿病缺血肢体的峰值血流量和平均血流量增加了约两倍。对后肢组织切片的形态学检查显示，一氧化碳水浴治疗后，糖尿病缺血动物的小动脉数量增加了约两倍。这些结果表明，一氧化碳水浴疗法可增加糖尿病缺血动物骨骼肌的血流量并促进血管生成，因此可能对治疗糖尿病外周动脉疾病有一定益处。
