Matsuoka I, Nakanishi H
Thromb Res. 1985 Jan 1;37(1):185-93. doi: 10.1016/0049-3848(85)90045-3.
Aggregation of washed rabbit platelets induced by arachidonic acid (AA) or collagen was inhibited by nitroprusside (NP) and 8-bromo cyclic GMP in a concentration-dependent manner. Although NP and 8-bromo cyclic GMP inhibited the AA-induced aggregation, these agents did not affect the conversion of exogenous AA to PG endoperoxides and TXA2 (which were observed as TXB2). On the other hand, collagen caused release of AA from phospholipids and sequential formation of TXB2 in [14C]AA prelabeled platelets. In contrast with the case in which exogenous AA was used, NP and 8-bromo cyclic GMP inhibited the collagen-induced formation of TXB2 by preventing the liberation of endogenous AA. These results indicate that cyclic GMP has at least two different inhibitory actions in platelets; one is the inhibition of AA release from phospholipids and the other is the inhibition of the action of TXA2 in platelets.
硝普钠（NP）和8-溴环鸟苷酸以浓度依赖的方式抑制花生四烯酸（AA）或胶原诱导的洗涤兔血小板聚集。尽管NP和8-溴环鸟苷酸抑制AA诱导的聚集，但这些药物并不影响外源性AA向内过氧化物和血栓素A2（以血栓素B2形式观察到）的转化。另一方面，胶原导致[14C]AA预标记血小板中AA从磷脂中释放并依次形成血栓素B2。与使用外源性AA的情况相反，NP和8-溴环鸟苷酸通过阻止内源性AA的释放来抑制胶原诱导的血栓素B2的形成。这些结果表明环鸟苷酸在血小板中至少有两种不同的抑制作用；一种是抑制AA从磷脂中释放，另一种是抑制血栓素A2在血小板中的作用。
