盖泽尔绵羊精原细胞的分离与增殖
Isolation and Proliferation of Spermatogonial Cells from Ghezel Sheep.
作者信息
Qasemi-Panahi Babak, Movahedin Mansoureh, Moghaddam Gholamali, Tajik Parviz, Koruji Mortaza, Ashrafi-Helan Javad, Rafat Seyed Abbas
机构信息
Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Anatomy, Faculty of Medical Science, University of Tarbiat Modares, Tehran, Iran.
出版信息
Avicenna J Med Biotechnol. 2018 Apr-Jun;10(2):93-97.
BACKGROUND
Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important breeds that are raised for meat, milk and wool. Field of spermatogonial cell technologies provides tools for genetic improvement of sheep herd and multiple opportunities for research. Spermatogonial cells are the only stem cells capable of transmitting genetic information to future generations.
METHODS
This study was designed to extend the technique of isolation and proliferation of spermatogonial cells in Ghezel sheep.
RESULTS
Isolated cells were characterized further by using specific markers for type A spermatogonia, including PLZF. Also, sertoli cells were characterized by vimentin which is a specific marker for sertoli cells. After 10 days of co-culture, viability rates of the cells was above 94.7%, but after the freezing process the viability rates were 74 percent.
CONCLUSION
In this study, a standard method for isolation and proliferation of spermatogonial stem cells in Ghezel sheep was developed.
背景
养羊业已采取措施向更高效、更具竞争力的领域转型。世界上有许多品种的绵羊，它们在不同文明的经济中都发挥着有益的作用。盖泽尔羊是伊朗的重要品种之一，用于产肉、产奶和产毛。精原细胞技术领域为羊群的遗传改良提供了工具，并带来了多种研究机会。精原细胞是唯一能够将遗传信息传递给后代的干细胞。
方法
本研究旨在扩展盖泽尔羊精原细胞的分离和增殖技术。
结果
利用A型精原细胞的特异性标记物（包括PLZF）对分离出的细胞进行了进一步鉴定。此外，支持细胞通过波形蛋白进行鉴定，波形蛋白是支持细胞的特异性标记物。共培养10天后，细胞活力率高于94.7%，但冷冻后活力率为74%。
结论
本研究建立了一种盖泽尔羊精原干细胞分离和增殖的标准方法。
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