Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Species Isolated from Soil.

spp. are predatory bacteria with great potential as antimicrobial agents. Studies have shown that members of the genus exhibit peculiar characteristics that influence their ecological adaptations. In this study, whole genomes of two different spp. designated SKB1291214 and SSB218315 isolated from soil were sequenced. The core genes shared by all the spp. considered for the pangenome analysis including the epibiotic were 795. The number of unique genes identified in spp. SKB1291214, SSB218315, W, and JJS was 1343, 113, 857, and 1572, respectively. These unique genes encode hydrolytic, chemotaxis, and transporter proteins which might be useful for predation in the strains. Furthermore, the two strains exhibited differences based on the % GC content, amino acid identity, and 16S rRNA gene sequence. The 16S rRNA gene sequence of sp. SKB1291214 shared 99% identity with that of an uncultured sp. clone 12L 106 (a pairwise distance of 0.008) and 95-97% identity (a pairwise distance of 0.043) with that of other culturable terrestrial spp., including strain SSB218315. In sp. SKB1291214, 174 bp sequence was inserted at the host interaction locus region usually attributed to prey attachment, invasion, and development of host independent phenotypes. Also, a gene equivalent to in HD100 was not conserved in sp. SKB1291214. The results of this study provided information on the genetic characteristics and diversity of the genus that can contribute to their successful applications as a biocontrol agent.