Isolation and characterization of a from fish pond water.

INTRODUCTION

The intricate habitats of aquatic organisms, coupled with the prevalence of pathogens, contribute to a high incidence of various diseases, particularly bacterial infections. Consequently, the formulation of sustainable and effective disease management strategies is crucial for the thriving aquaculture sector.

METHODS AND RESULTS

In this investigation, a strain of , designated , was isolated from a freshwater fish pond. Identification was achieved through microscopic examination of morphological characteristics, biochemical property assessment, and phylogenetic analysis. The lysogenic capability of was evaluated, revealing its effectiveness in lysing , , , , and . Physiological analysis indicated that the optimal ratio of to host bacteria was 1:10,000, with strict aerobic requirements. The optimal pH range for growth and reproduction was 7.0-8.0, the ideal temperature was found to be 30-35°C, with a preferred Na concentration of 0% and a Ca concentration of 15-25 mM. Additionally, demonstrated enhanced lytic activity against bacteria in aquaculture effluent while effectively managing ammonia-nitrogen levels.

DISCUSSION

In summary, holds significant promise for development as a probiotic agent in aquaculture.