Wong Hoi-Shan, Monternier Pierre-Axel, Orr Adam L, Brand Martin D
Buck Institute for Research on Aging, Novato, CA, USA.
Helen and Robert Appel Alzheimer's Disease Research Institute, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
Methods Mol Biol. 2018;1782:287-299. doi: 10.1007/978-1-4939-7831-1_16.
Superoxide and hydrogen peroxide produced by mitochondria play important roles in various physiological and pathological processes. This chapter describes a plate-based method to measure rates of superoxide and/or hydrogen peroxide production at specific sites in isolated mitochondria.
线粒体产生的超氧化物和过氧化氢在各种生理和病理过程中发挥着重要作用。本章介绍一种基于平板的方法,用于测量分离线粒体中特定部位超氧化物和/或过氧化氢的产生速率。