Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA.
Methods Mol Biol. 2023;2675:77-96. doi: 10.1007/978-1-0716-3247-5_7.
Methods for isolating mitochondria from different rodent tissues have been established for decades. Although the general principles for crude mitochondrial preparations are largely shared across tissues - tissue disruption followed by differential centrifugation - critical differences exist for isolation from different tissues to optimize mitochondrial yield and function. This protocol offers a unified resource for preparations of isolated mitochondria from mouse liver, kidney, heart, brain, skeletal muscle, and brown and white adipose tissue suitable for functional analysis.
几十年来，已经建立了从不同啮齿动物组织中分离线粒体的方法。虽然粗线粒体制备的一般原则在很大程度上在组织间共享 - 组织破坏后进行差速离心 - 但从不同组织中分离以优化线粒体产量和功能时，存在关键差异。本方案提供了一种从小鼠肝、肾、心、脑、骨骼肌以及棕色和白色脂肪组织中分离分离线粒体的统一资源，适用于功能分析。
